Effects of enzymatic hydrolysates from mantle of pearl oyster Pinctada martensii on wound healing of mouse skin
YANG Fa-ming;LIN Hai-sheng;QIN Xiao-ming;ZHANG Chao-hua;CAO Wen-hong;GAO Jia-long
College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China;Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety, Key Laboratory of Advanced Processing of Aquatic Products of Guangdong Higher Education Institution, National Research and Development Branch Center for Shellfish Processing (Zhanjiang), South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Zhanjiang 524088, China
Abstract: SPF male mice were randomly divided into negative control group, positive control group and experimental groups(low, medium and high), and the mice with incision dorsal wound model were intragastrically administrated by enzymatic hydrolysis from mantle of pearl oyster Pinctada martensii(EHM) continuously for 14 days. The wound healing rate and scar reduction rate of soft tissue were measured every other day and biochemical factors including interleukin 6(IL-6), interleukin 10(IL-10), transforming growth factor-β(TGF-β), fibroblast growth factor 2(FGF-2), epidermal growth factor(EGF), cyclin D1(CCND1) and hydroxyproline were investigated in marginal tissue of wound of the mice at the 3 rd day, 7 th day, and 14 th day to evaluate the effects of EHM on wound healing of skin in mouse. Results showed that the EHM led to improve the healing rate and scar shrinkage of wound in mouse compared with the negative control group at 6 th and 10 th day(P<0.05). The EHM was shown to effectively increase the protein expression of anti-inflammatory cytokine IL-10(P<0.01) in wound tissues, without significant effect on inflammatory factor IL-6(P>0.05). There were significantly higher contents of TGF-β, EGF and CCND1 in injured skin tissue in the high dose group than those in control group(P<0.05), without significant differencein content of FGF-2 between the test groups(P>0.05). On the 14 th day, hydroxyproline content in the skin tissue was significantly increased in each EHM groups(P<0.05). The findings indicated that EHM could inhabit inflammation, promote growth factors and collagen production, thus accelerate open wound healing of soft tissue in mice, and that EHM has a certain inhibitory effect on superficial scar hyperplasia.
杨发明, 林海生, 秦小明, 章超桦, 曹文红, 高加龙. 珍珠贝外套膜酶解产物促进小鼠皮肤创伤愈合作用研究[J]. 大连海洋大学学报, 2019, 34(4): 492-498.
YANG Fa-ming, LIN Hai-sheng, QIN Xiao-ming, ZHANG Chao-hua, CAO Wen-hong, GAO Jia-long. Effects of enzymatic hydrolysates from mantle of pearl oyster Pinctada martensii on wound healing of mouse skin. Journal of Dalian Ocean University, 2019, 34(4): 492-498.
2019, Vol. 34 
