Transcriptome analysis of skin of red and white koi carp Cyprinus carpio haematopterus
SHI Dong-jie;HU Jin-you;WANG Sai-sai;ZHU Hua;ZHANG Xin;ZHU Li-fei;SUN Yan-sheng
National Engineering Research Center for Freshwater Fisheries, Key Laboratory of Urban Agriculture (North),Ministry of Agriculture and Rural Affairs, Beijing Key Laboratory of Fisheries Biotechnology, Beijing Fisheries Research Institute, Beijing 100097, China;College of Engineering, China Agricultural University, Beijing 100083, China
Abstract: Illumina PE150 high-throughput sequencing technology was used to sequence the transcriptome of white skin and red skin tissue of Cyprinus carpio haematopterus with body length of(28.34±3.12)cm in order to further develop gene resources of red and white koi carp Cyprinus carpio haematopterus. The 116.26 Gb Clean Date obtained were compared with seven databases of Nr, GO, KEGG, KOG, Pfam, Swiss-Prot, and eggNOG. A total of 10 476 annotated genes were obtained, with the maxiaml percent of annotation success(35.62%) in Nr database. The functional and pathway enrichment analysis revealed that differential genes were enriched in 132 metabolic pathways including regulation of actin cytoskeleton and focal adhesion pathway. qRT-PCR of 9 differentially expressed genes selected randomly showed that the expression of differentially expressed genes in the skin of Cyprinus carpio haematopterus was consistent with the quantitative results of RNA-seq, indicating that the transcriptome sequencing were reliable. The transcriptome data obtained in this study can provide basic data for future research on key gene development and functional analysis of ornamental fish skin color.
史东杰, 胡金有, 王赛赛, 朱华, 张欣, 朱莉飞, 孙砚胜. 红白锦鲤皮肤转录组测序分析[J]. 大连海洋大学学报, 2019, 34(4): 475-481.
SHI Dong-jie, HU Jin-you, WANG Sai-sai, ZHU Hua, ZHANG Xin, ZHU Li-fei, SUN Yan-sheng. Transcriptome analysis of skin of red and white koi carp Cyprinus carpio haematopterus. Journal of Dalian Ocean University, 2019, 34(4): 475-481.
2019, Vol. 34 
