Abstract: In the present study,the expressions of three Toll receptor genes TLR3,TLR7 and TLR22 were investigated in gill,spleen,hepatopancreas,intestine and head kidney in grass carp Ctenopharyngodon idella intraperitoneally injected with bacterial pathogen Flavobacterium columnare G41,4 and 7 days after injection by semi-quantified RT-PCR which were analyzed by electrophoresis on 12 g/L TAE agarose gels.The densitometry was used by Quantity One 4.62,and in the control group,the fish were injected intraperitoneally with sterile phosphate buffer solution(PBS).The results showed that the expression of TLR3 gene was found to be significantly increased in the gill,hepatopancreas,intestine and head kidney 7 days after injection compared with the fish in the control group, and the expressions of TLR7 and TLR22 genes were significantly upregulated in 5 different tissues 4 and 7 days after injection.The expression of TLR22 gene was increased significantly in gill and spleen and hepatopancreas compared with the control fish only 1 day after injection,indicating that TLR3,TLR7 and TLR22 gens are effectively activated after administration by the pathogen F.columnare.
2013, Vol. 28 
