1.Wuxi Fisheries College，Nanjing Agricultural University，Wuxi 214081，China；2.Laboratory of Risk Assessment of Environmental Factors for Aquatic Product Quality，Safety and Environment，Ministry of Agriculture and Rural Affairs (Wuxi)，Key Laboratory of Inland Fishery Ecology and Environment，Chinese Academy of Fishery Sciences，Freshwater Fisheries Research Center，Chinese Academy of Fishery Sciences，Wuxi 214081，China；3.Kunshan Fisheries Technology Promotion Station，Kunshan 215399，China
Abstract: To improve ozone disinfection process and optimize high efficiency disinfection technology of tailwater in aquaculture, different concentrations of ozonated water were prepared by regulation of generation period of an ozone generator, concentration and exposure period (1 and 10 min) of ozone neutralizer, and the sterilizing effect of ozonated water against different concentrations of Aeromonashydrophila was determined by suspension quantification method under different stress times. The sterilizing effect was compared with the conventional disinfectant potassium persulfate. The results showed that the sterilization rate was found to be 92.66% at 6.4×105CFU/mL, 84.00% at 7.5×106CFU/mL, 80.67% at 1.5×107 CFU/mL and 79.58% at 3.2×108 CFU/mL with 1.2 mg/L ozone for 2 min, and 100%, 99.72%, 99.96% and 99.98% for 6 min, while the sterilization rate was 100% when potassium persulfate at 400 mg/L was used for 6 min, and the actual effects of ozone treatment of bacterial culture tail water were also tested. The findings indicated that the sterilization efficiency of ozone was much higher than that of potassium persulfate, with the optimal concentration of 1.2 mg/L, and the action time of 6 min, indicating that ozone sterilization rate for A.hydrophila was correlated with number of bacteria, concentration of ozone, and active time.
2023, Vol. 38 
