Analysis of bacterial community compositions in three biological filter media by 16S rRNA gene library
LI Qian1，2，HU Ting-jian1，2，XIN Jian-mei1，WANG Yu-chen1，ZHOU Zhi-ming1
1.Zhejiang Institute of Freshwater Fisheries，Huzhou 313001，China；2.Key Laboratory of Healthy Freshwater Aquaculture，Ministry of Agriculture，Huzhou 313001，China
Abstract: The present study was aimed to analyze the composition of bacteria community and to evaluate the applications of biological filter media in recirculating aquaculture system.Bio-filters were constructed by three types of media and the bacterial community compositions of bio-film as well as water samples were studied using 16S rRNA gene amplification and Illumina high-throughput sequencing platform.The results showed that the three media all enriched the bacterial abundance and diversity，and that there were more bacterial species and higher biodiversity in the three media than those in the water samples.The predominant bacterial phylum was the same Firmicutes in all water samples of the three bio-filters.On the three media，however，there were different predominant bacteria in bio-film，including Firmicutes in polyethylene ball medium and Proteobacteria in submerged ceramics medium and elastic brush medium.In terms of bacterial species and diversity，the maximal value（26 genera）was observed in elastic brush medium.However，23 genera，the minimal species composition，was detected in polyethylene ball medium.
李倩, 胡廷尖, 辛建美, 王雨辰, 周志明. 应用16SrRNA基因文库技术分析3种生物填料上生物膜的细菌群落组成[J]. 大连海洋大学学报, 2016, 31(4): 384-389.
LI Qian, HU Ting-jian, XIN Jian-mei, WANG Yu-chen, ZHOU Zhi-ming. Analysis of bacterial community compositions in three biological filter media by 16S rRNA gene library. Journal of Dalian Ocean University, 2016, 31(4): 384-389.
2016, Vol. 31 
