RAPD Analysis of the polymorphism of genomic DNA among three abalones
XING Rong lian1, DING Jun1, TENG Liping2, CHANG Ya qing1, SONG Lin shen3
Dalian Fisheries Univ., Dalian 116023, China;2.Dalian Laohutan Ocean Park, Dalian 116013, China; 3.Experimental Marine Biology Laboratory Institute of Oceanology,Chinese Academy of Sciences, Qingdao 266071, China
Abstract: Polymorphism of genomic DNA and molecular classification of three species of abalones, Haliotis midae, Haliotis discus hannai, Haliotis diversicolor supertexte，were determined by random amplified polymorphic DNA (RAPD). Amplifications with 24 primers gave 376 reproducible amplified fragments ranging between 250 and 2500 bp in length，the amplified fragments were scored as present（1） or absent（0） for each DNA sample and an index of degree of band sharing（F） was calculated by Nei and Lis matching coefficient method. The value of（1-F） was used to evaluate genetic distances between species. The phylogenetic trees were constructed with the methods of UPGMA and NJ on the basis of genetic distances. UPGMA analysis indicated that there was a close relationship between H. midae and H. diversicolor supertexte, followed by H. discus hannai, and no significant genetic distances between the abalones. RAPD approach provides a new method for efficient and simple classification and identification of new strains for abalones.
邢荣莲, 丁君, 滕利平, 常亚青, 宋林生. 3种鲍基因组DNA多态性的RAPD分析[J]. 大连海洋大学学报, 2003, 18(3): 191-196.
XING Rong lian, DING Jun, TENG Liping, CHANG Ya qing, SONG Lin shen. RAPD Analysis of the polymorphism of genomic DNA among three abalones. Journal of Dalian Ocean University, 2003, 18(3): 191-196.
2003, Vol. 18 
