Abstract: The spermatozoon cryopreservation were conducted in sea urchin Strongylocentrotus intermedius.The higher pose-thawed sperm survival rate(58.2%)and fertilization rate(24.7%)were observed in the cryoprptectant comprised of natural sea water diluted with 10%DMSO,6%glycerin and 25 mmol/L trehalose as the cryoprotector and mixed with sperm at a ratio of 1∶1,equilibration at 4℃for 15 min,swung at 15 cm above LN2for 3 min and then 3 cm above LN2for 8 min,finally transferred into LN2for storage.When the post-thawed sperm was fertilized,the seawater were added to the sperm at a rate of 0,28,56,112 mmol/L,and 280 mmol/L glucose.There was higher fertilization(26%)in the sperm supplemented with 56 mmol/L glucose than that in the other groups, indicating that freezing method,cryoprptectant and equilibration time all had influence on cryopreservation of the spermatozoa in the freezing process.The survival rate and fertilization rate of the pose-thawed sperm were greatly improved through various factor optimization such as addition of appropriate glucose.
2012, Vol. 27 
