Abstract: Thecfbgene ofStreptococcusagalactiaewas amplified by a set of four specific primers that recognize six distinct sequences of the target in 40 min by incubating all of the reagents in a single tube with BstDNA polymerase at a constant 65 ℃. A loop-mediated isothermal amplification (LAMP) assay was developed and validated for the specific detection ofStreptococcusagalactiae, with detection limits of 3.7×101-3.7×102cfu/mL. The resulting amplicons were visualized by adding calcein to the reaction tube before the reaction. FTA cards was used for collection and purification of nucleic acids from a liver of green terrorAequidensrivulatuswith pathogenic bacteria in field applications. The findings demonstrate that the LAMP-based assay is a sensitive and reliable means for the detection ofStreptococcusagalactiaewith rapidity, simplicity and low cost, especially suitable for on-site rapid diagnosis.
2014, Vol. 29 
