Abstract: Early diagnosis is pivotal for disease control of aquaculture animals. Previously, physiological and biochemical reactions and serological tests have been applied for disease diagnosis. However, it is difficult to diffuse and adopt these methods due to time and expertise required. With the rapid development of molecular biological technique, polymerase chain reaction and probe-based nucleic acid detection have been used widely in disease diagnosis of aquatic animals. The loop-mediated isothermal amplification as an efficient, simple and specific nucleic acid amplification technique can detect viral, bacterial, parasitic, and fungal diseases in both plants and animals,and has been used in detection of pathogen in aquatic animals since it was established. According to previous studies, quite a lot of aquatic pathogens including Iridovirus, Herpesvirus, Edwardsiella, Vibrio, Myxosporea, Clonorchissinensis have been detected effectively using this technology. This technology is considered to be of great development prospect, and has been continuously improved and widely used in the pathogen detection of aquaculture animals in recent years.
潘奕如, 曹景龙, 王静远, 黎洁. 环介导等温扩增技术在水产病害检测中的研究与应用[J]. 大连海洋大学学报, 2018, 33(4): 539-550.
PAN Yi-ru, CAO Jing-long, WANG Jing-yuan, LI Jie, . Research and application of Loop-mediated isothermal amplification in detection of aquaculture diseases：a review. Journal of Dalian Ocean University, 2018, 33(4): 539-550.
2018, Vol. 33 
