Screening and distribution of important pathogenic bacteria in different medium of typical recreational beaches in Dalian
BI Hong-yu1，2, MING Hong-xia2, MA Yue-xin1, SU Jie2, SUN Zhi-hao1，2, WANG Yan-tao1，2, JIN Yuan2, FAN Jing-feng2
1.College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China; 2.National Marine Environmental Monitoring Center, Dalian 116023, China
Abstract: The pathogenic bacteria were screened in surface seawater, sediment, wet sand and dry sand at Dalian Jinshitan Bathing Beach and Fujiazhuang Bathing Beach by culturable, gene chip and high-throughput sequencing technologies， and the spatial distribution characteristics of indicator bacteria and common pathogenic bacteria were analyzed to investigate the species and distribution of pathogenic bacteria in the beach. High-throughput sequencing results showed that the dominant genus of both the Jinshitan and Fujiazhuang bathing beaches were Vibrio, Bacillus, Pseudomonas and possible pathogens of which were some species in Vibrio, Pseudomonas, and Klebsiella. The gene chip detection revealed that the main pathogenic bacteria in these two beaches were V.alginolyticus, V.parahaemolyticus, V.harveyi, V.anguillarum, Enterococcusfaecalis, Aeromonas, Shigella and Escherichiacoli, which posed a huge potential threat to the health of entertainers. Analysis of spatial distribution indicated that fecal indicator bacteria, fecal coliforms and Enterococcus were mainly distributed in seawater and dry sand, among all sites, with the maximal concentration of pathogenic bacteria in the sewage outlet site. The potential pathogenic bacterium Staphylococcusaureus was primarily found in seawater and dry sand, and the cultarable Vibrio was mainly observed in seawater. Since the sewage outlet is the main source of pathogenic bacteria in Dalian Jinshitan and Fujiazhuang beaches as an important non-point source pollution, it is recommended that pathogenic bacteria around beaches and sewage outlets should be regularly monitored during the peak of swimming season.
毕宏宇, 明红霞, 马悦欣, 苏洁, 孙志浩, 王艳涛, 金媛, 樊景凤. 大连典型娱乐海水浴场重要病原细菌筛选及其在不同介质中的分布[J]. 大连海洋大学学报, 2019, 34(2): 204-213.
BI Hong-yu, MING Hong-xia, MA Yue-xin, SU Jie, SUN Zhi-hao, WANG Yan-tao, JIN Yuan, FAN Jing-feng. Screening and distribution of important pathogenic bacteria in different medium of typical recreational beaches in Dalian. Journal of Dalian Ocean University, 2019, 34(2): 204-213.
2019, Vol. 34 
