Abstract: Simulation research on bioaccumulation of 3,4-benzo[a]pyrene, 9,10-dimethyl anthracene, and 3-methyl phenanthrene was conducted in sea urchinStrongylocentrotusintermedius with shell diameter of (2.0±0.3)cm by a semi-static two-compartment kinetic model to study bioconcentration kinetics of PAHs in sea urchin and to understand the dynamic parameters including absorption rate constant (K1), release rate constant (K2), biological enrichment factor (BCF), content (Cmax) and biological half-life (t1/2) of the three PAHs in the sea urchin in the equilibrium state by nonlinear fitting. The kinetic parameter average values in the sea urchin were shown to be 59.1 inK1, 0.152 inK2, 136.5 inBCF, 11.788 μg/g inCmax, and 6.36 d int1/2 for 3,4-benzo[a]pyrene; 12.3 inK1, 0.228 inK2, 54.05 inBCF, 4.383 μg/g inCmax, and 6.32 d int1/2 for 9, 10-dimethyl anthracene; and 18.16 inK1, 0.113 inK2, 88.1 inBCF, 12.407 μg/g inCmax, and 7.6 d int1/2 for 3-methyl phenanthrene. The descending order ofBCF of the three PAHs absorbed by sea urchin was expressed as 3,4-benzo[a]pyrene (14 dBCF=220)>3-methyl phenanthrene (14 dBCF=128.4)>9,10-dimethyl anthracene (14 dBCF=84.0) in the sea urchin exposed to the concentration of 5 μg/L for the three PAHs, indicating that the absorption processes of PAHs in sea urchin conform to two-compartment kinetic model for 3,4-benzo[a]pyrene, 9,10-dimethyl anthracene and 3-methyl phenanthrene.