Effects of total ammonia nitrogen content on early growth and development of Manila clam Ruditapes philippinarum
HAN Tian－kun1， YANG Feng1，2， JIA Jia1， LI Xiao－xu1， YAN Xi－wu1，2
1.College of Fisheries and Life Science， Dalian Ocean University， Dalian 116023， China； 2.Engineering Research Center of Shellfish Culture and Breeding in Liaoning Province， Dalian 116023， China
Abstract: Acute toxicity test of total ammonia nitrogen(TAN)level(0.167－29.6 mg/L， non－ionic ammonia，UIA， 0.012－2.03 mg/L)on fertilized eggs， D－larvae with body length of(103.2±3.0)μm and juveniles with body length of(318.1±27.3)μm in Manila clam Ruditapes philippinarum was conducted at water temperature of 21－24℃， pH 7.9－8.3， and a salinity of 27－30 in order to investigate the tolerance of Manila clam to TAN at early development.It was found that the 24 h effective concentration(EC50)of TAN on hatching rate was 7.29 mg/L(UIA 0.502 mg/L)in fertilized eggs and 96 h half lethal concentration(96 h LC50)was 7.94 mg/L(UIA 0.212 mg/L)for D－larvae and 49.0 mg/L(UIA 2.10 mg/L)for juveniles.The 96 h EC50was 4.9 mg/L(UIA，0.21 mg/L)for relative growth of juveniles for shell length and 10.5 mg/L(UIA 0.448 mg/L)for shell height.The order of tolerance of Manila clam to ammonia nitrogen level was expressed as juvenile＞ D－larvae， suggesting that UIA should be controlled at below 0.020 mg/L during breeding.
韩天坤, 杨凤, 贾甲, 李晓旭, 闫喜武. 总氨态氮对菲律宾蛤仔早期生长发育的影响[J]. 大连海洋大学学报, 2018, 33(2): 210-216.
HAN Tian－kun, YANG Feng, JIA Jia, LI Xiao－xu, YAN Xi－wu, . Effects of total ammonia nitrogen content on early growth and development of Manila clam Ruditapes philippinarum. Journal of Dalian Ocean University, 2018, 33(2): 210-216.
2018, Vol. 33 
