Changes in biochemical composition of Manila clam Ruditapes philippinarum before and after overwintering
YANG Dong－min1， WANG Hua－min1， DING Jian－feng1，2， YANG Feng1，2， YAN Xi－wu1，2
1.College of Fisheries and Life Science， Dalian Ocean University， Dalian 116023， China； 2.Engineering Research Center of Shellfish Culture and Breeding in Liaoning Province， Dalian 116023， China
Abstract: The contents of hydration water， glycogen， glycerol， fat， fatty acid and amino acid were determined in wild Manila clam Ruditapes philippinarum with body weight of(15.45±1.00)g collected from Jinshitan Bay in northern China in December(pre wintering)and in March(post wintering)to study cold resistance mechanism of Manila clam.The results showed that there were significantly lower contents of glycogen and fat and significantly higher glycerol levels in clams after overwintering(p＜0.05).The contents of fatty acid including C18:0， C20:4n－6，C22:5n－6and C22:5n－3were significantly decreased after winter(p＜0.05)， while the content of C20:5n－3(EPA)was significantly increased after winter(p＜0.05).The contents of amino acid including isoleucine， glutamic acid and lysine were significantly increased in the clam after overwinter(p＜0.05).These findings indicated that there was a close relationship between the clam biochemical composition and cold resistance，and that provide theoretical reference for the winter management of clam.
2017, Vol. 32 
