Abstract: Effects of several Chinese herbal medicines on immune ability of 1+old Amur sturgeonAcipenser schrenckiiBrandtwere investigated at normal and high temperature to screen anti-heat stress Chinese herbalmedicines in Amur sturgeon culture.Amur sturgeon were divided into 4 groups at a density of30 fish in one group and orally administrated by Chinese herbalmedicinesAngelica sinensis,Houttuyniacordataand prescription IV(PIV)at a dose of0(distilled water,as control group),0.30,0.54 g/kg or0.80 g/kg body weightonce a day for14 d with duplication.After the withdrawal of 24 h,half of the fish in each group were thermally stimulated at30℃for 2 h and then the bloodswere sampled together with the normal temperature groups at22℃.The protein contents,cell phagocytosis in serum and lysozyme activities in several tissues were detected.The results showed that PIV andHouttuyniacordataeffectively inhibited the increase in serum protein levels in the fish exposed to the heat stress. The phagocytosis function was improved in the fish under normal temperature byAngelica sinensis,while PIV led to effectively increase the cell phagocytosis,and to effectively improve the lysozyme activities in liver,serum,gill and stomach and homeostasis in the fish under high temperature.In summary,it is conferred that PIV effectivelymaintains homeostasis and promotes cell phagocytosis and lysozyme activity,being used as the anti-heat stress Chinese herbalmedicine for further study.
王荻, 刘红柏. 施氏鲟抗热应激中草药免疫制剂的筛选[J]. 大连海洋大学学报, 2016, 31(6): 635-639.
WANG Di, LIU Hong-bai. Screening of Chinese herbalmedicines related to anti-heat stress in Amur sturgeonAcipenser schrenckiiBrandt. Journal of Dalian Ocean University, 2016, 31(6): 635-639.