1.International Research Center for Marine Biosciences，Ministry of Science and Technology，Shanghai Ocean University，Shanghai 201306，China；2.Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources，Ministry of Education，Shanghai Ocean University，Shanghai 201306，China；3.Shanghai Collaborative Innovation Center for Cultivating Elite Breeds and Green-Culture of Aquaculture Animals，Shanghai 201306，China
Abstract: To investigate the effects of outer membrane vesicles (OMVs) on the biofilm formation of Pseudoalteromonasmarina and the settlement of mussel (Mytiluscoruscus) plantigrades with shell length (0.88 mm±0.16 mm) and shell height of (0.55 mm±0.12 mm), OMVs were extracted and purified from P.marina and added into P.marina bacterial fluid to form biofilms, and the inducing capacity of biofilms on the settlement of mussel juveniles was detected at the final OMVs concentration of 0.1, 1, 10 and 20 μg/mL. The results showed that OMVs were successfully extracted from P.marina, with the more content of OMVs extracted from the bacterium than that in the supernatant. The biofilms had the maximal inducing activity to mussel plantigrades, with the settlement rate of 56.42% at the OMVs concentration of 10 μg/mL. With the increase in OMVs concentration, the bacterial density and biofilm thickness of biofilms were found to be increased, with increase in α-polysaccharides, β-polysaccharides, lipids and proteins in extracellular products. Correlation analysis showed that the lipid content in the biofilms was significantly correlated with mussel settlement (P<0.05), indicating that addition of OMVs promoted the formation of P.marina biofilms and increases the content of extracellular products of biofilms. It is speculated that the improvement in inducing ability of biofilmd to mussel settlement after adding OMVs is primarily due to the increase in lipid content.
2023, Vol. 38 
