Effects of three fatty acids on biofilm formation and settlement and metamorphosis of hard shelled mussel Mytilus coruscus
LIANG Xiao, DING Wenyang, ZHANG Chi, CAI Yushan, YANG Jinlong
1.International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai 201306, China; 2.Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China; 3.Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou)，Guangzhou 511458, China
Abstract: The study was designed to evaluate the influence of fatty acids on the biofilm formation of Pseudoalteromonasmarina and the co-formed biofilm on settlement and metamorphosis of the hard shelled mussel Mytiluscoruscus.The palmitic acid, stearic acid, oleic acid and mixture of these three fatty acids(1∶1∶1 in mass ratio)was added into the bacterial solution with final density of 5×108 cells/mL at a dose of 0(control group), 0.01, 0.1, 1 and 5 mg/L to form a biofilm to probe into the density, biofilm thickness, and extracellular products of biofilms, and then the influence of the formed biofilm on the settlement and metamorphosis rate of the larval hard shelled mussel was investigated in a 20 mL sterile seawater petri dish stocked with 20 individuals of pediveliger larvae.The results showed that the biofilm formed by the mixed fatty acids and the bacteria led to significantly increase the rates of settlement and metamorphosis of the larvae.The analysis of the biological characteristics of the biofilm revealed that the bacterial density was significantly reduced, the bacteria were more dispersed, and the film thickness was significantly reduced compared with the biofilm of P.marina with the increase in the concentration of the three fatty acids mixtures(P<0.05).There was significantly increase in extracellular lipids(P<0.05), without significant difference in extracellular polysaccharides and extracellular proteins(P>0.05).The findings indicated that fatty acids affected larval settlement and metamorphosis of the hard shelled mussel by promoting the production of biofilm extracellular lipids.
梁箫, 丁文扬, 张驰, 蔡雨珊, 杨金龙. 3种脂肪酸对生物被膜形成及厚壳贻贝幼虫附着变态的影响[J]. 大连海洋大学学报, 2021, 36(4): 580-586.
LIANG Xiao, DING Wenyang, ZHANG Chi, CAI Yushan, YANG Jinlong. Effects of three fatty acids on biofilm formation and settlement and metamorphosis of hard shelled mussel Mytilus coruscus. Journal of Dalian Ocean University, 2021, 36(4): 580-586.
2021, Vol. 36 
