Effects of alginate on biofilm formation of Pseudoalteromonas marina and larval settlement and metamorphosis of the mussel Mytilus coruscus
CHEN Huie, HE Kang, HE Chuhan, ZHU Youting, YANG Jinlong*, LIANG Xiao*
1.International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai 201306, China;2.Shanghai Collaborative Innovation Center for Cultivating Elite Breeds and Green-Culture of Aquaculture Animals, Shanghai 201306, China; 3.Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Guangzhou 511458, China
Abstract: In order to research effects of alginate on biofilm formation of bacterium Pseudoalteromonasmarina and the roles of co-formed biofilm on larval settlement and metamorphosis of larval mussel Mytiluscoruscus, the settlement and metamorphosis of the mussel larvae were investigated on biofilm of P.marina as a control, and the bacterial biofilm containing alginate solution at final concentration of 0.1, 0.5, 1.0, 1.5 and 2.0 mg/L. In addition, the optimal concentration of alginate was selected to compare and analyze the changes in density, thickness and extracellular products of the biofilm. The bacterial density on the biofilms was shown to be increased with the increase in the alginate concentration. There were significantly increase in thickness and extracellular polysaccharide contents of the biofilm at alginate concentration of 0.5 mg/L (P<0.05), and finally increase in biofilm-inducing activities. The findings have positive significance for understanding of the induction mechanism of marine shellfish larval settlement and metamorphosis, and provide theoretical basis for the use of biofilms to improve the success rate of mussel seedlings. The appropriate concentration of alginate is 0.5 mg/L.
陈慧娥, 贺康, 贺楚晗, 竹攸汀, 杨金龙, 梁箫. 藻酸盐对海假交替单胞菌生物被膜形成及厚壳贻贝附着变态的影响[J]. 大连海洋大学学报, 2022, 37(4): 620-626.
CHEN Huie, HE Kang, HE Chuhan, ZHU Youting, YANG Jinlong, LIANG Xiao. Effects of alginate on biofilm formation of Pseudoalteromonas marina and larval settlement and metamorphosis of the mussel Mytilus coruscus. Journal of Dalian Ocean University, 2022, 37(4): 620-626.
2022, Vol. 37 
