Abstract: In order to study the organisms attachment of 3D printed concrete artificial reef, the species and biomass of attached organisms were comparatively investigated on 3D printed hanging plate specimens with nozzle diameters of 20 mm (3D-20 group) and 10 mm (3D-10 group) and cast-in-place hanging plate specimens with the same mix proportion (PC group)disposed in Heishijiao sea area of Dalian, Liaoning Province on November 1, 2020 (for 2 months), February 1, 2021 (for 3 months), and July 1, 2021(for 5 months). After the 1st and 2nd sampling, the specimens were put back in place. The offshore hanging board were completed on September 1, 2020. A total of 17 species of attached organisms were sampled and identified, with dominant species snail Littorinabrevicula in each sampling. The total biomass was shown to be 561.58 g/m2 in 3D-20 group, 215.72 g/m2 in 3D-10 group, and 203.24 g/m2 in PC group in the third sampling. For three samplings, there was significantly higher total biomass in 3D-20 group than that in 3D-10 group and PC group, significant difference between 3D-20 group and 3D-10 group and PC group (P<0.05), and no significant difference between 3D-10 group and PC group (P>0.05). The finding indicates that 3D printed concrete is practical as artificial reef material, and the roughness of reef surface caused by printing method is superior to biological attachment.