Impacts of heavy metals and environmental factors assisted antibiotics on antibiotic resistance genes in sediments in Dalian typical intertidal mudflat culture areas
LIU Xuan1，ZHAO Huimin1，WANG Hua2*，LIU Lu2,3，WANG Shihui2，SUN Qi2
1.School of Environment, Dalian University of Technology, Dalian; 2.College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China; 3.Chinese Academy of Fishery Sciences，Beijing
Abstract: Intensive aquaculture areas are the important reservoirs for antibiotic resistance genes(ARGs)in water environment.Existence and abundance of 15 antibiotic resistance genes(ARGs)and 2 integrons in the sediments were monitored in one of the typical intertidal mudflat culture areas at coastal Pikou in Dalian, by Real-time quantitative PCR technology, and the impacts of antibiotics, heavy metals, and physicochemical characteristics on ARGs were also investigated in March, June, September and November 2016 to explore the influences of antibiotics, heavy metals, and environmental factors on ARGs in aquaculture area.Results showed that there were 13 ARGs and 2 integronst in the sediments of coast culture areas in the intertidal mudflat culture area in 2016, with concentrations[lg(copies/g)]ranging from 1.57 to 5.94, and with dominant ARGs qnrA, qnrB, qnrS, sul1, sul2, floR, and fexA.Annual concentration range in the sediments was changed between 8.10 and 8.69 in pH, 25.1 and 26.7 in salinity, 8.602 g/kg and 10.580 g/kg in total nitrogen, 0.335 g/kg and 0.482 g/kg in total phosphorus, 1.659 g/kg and 1.792 g/kg in total ammonia nitrogen, and 16.447 g/kg and 16.666 g/kg in total organic carbon.The metal concentration(mg/kg)in the sediments was varied within 16.456-23.509 in Cu, 60.141-93.478 in Zn, 1.140-1.740 in Cr, 0.120-0.143 in Cd, 23.274-27.075 in Pb, and 0.019-0.037 in Hg.A total of 15 kinds of antibiotics were detected in the sediments, with the dominant antibiotics of fluoroquinolones(FQs)in all tested antibiotics, and all 6 special types of FQs were detected, with the total concentration ranges of 4.682-78.368 ng/g.Person correlation analysis suggested that antibiotics play the indirectly selection and cross-selection roles in the occurrence and distribution of ARGs, with very significantly positive correlation between heavy metals Cu and Zn contents and the abundance of ARGs(r=0.676-0.784, P<0.01), and significantly negative correlation between pH, salinity, Cr level, and Pb level and ARGs abundance(r=-0.897--0.696, P<0.05 or P<0.01).Canonical correspondence analysis indicated that the co-selection roles of antibiotics coupled with various heavy metals and environmental factors were the pivotal factors for the presence and distribution of ARGs in the sediments of in the intertidal mudflat culture area.The findings indicated that qnrS and sul1 were used as indicator genes for ARGs contamination in the typical coast culture environment.
刘萱, 赵慧敏, 王华, 刘璐、, 王诗慧, 孙琦. 大连典型滩涂养殖区沉积物中抗生素协同重金属和环境因素对抗性基因分布的影响[J]. 大连海洋大学学报, 2020, 35(2): 229-238.
LIU Xuan, ZHAO Huimin, WANG Hua, LIU Lu, WANG Shihui, SUN Qi. Impacts of heavy metals and environmental factors assisted antibiotics on antibiotic resistance genes in sediments in Dalian typical intertidal mudflat culture areas. Journal of Dalian Ocean University, 2020, 35(2): 229-238.
2020, Vol. 35 
