Abstract: The ZnO photocatalysts were successfully prepared and characterized by XRD and SEM analysis and then employed in the photocataltic treatment of oxytetracycline hydrochloride pollution in order to eliminate oxytetracycline hydrochloride in aquaculture effluent scientifically and effectively. The effects of ZnO calcination temperature, calcination time, dosage, concentration of H2O2, reaction time and initial concentration of oxytetracycline hydrochloride on the photocatalytic effect were investigated under UV light conditions, and the ZnO photocatalystic conditions were optimized through an orthogonal experiment. The results showed that there was good elimination of oxytetracycline hydrochloride wastewater using the nano-ZnO photocatalysts under the optimal conditions of the oxytetracycline concentration of 0.01 g/L,the calcination of nano-ZnO at 450 ℃, for 1 h calcination, mass concentration of 0.8 g/L, H2O2concentration of 0.2 g/L and reaction period for 3.5 h, with an average removal rate of 75.45% for oxytetracycline hydrochloride in aquaculture wastewater. The findings indicate that nano-ZnO can be used in production practice since it has high removal rate of oxytetracycline hydrochloride in aquaculture effluent without secondary pollution.