Journal of Dalian Ocean University  2024, Vol. 39 Issue (1): 38-47 DOI: 10.16535/j.cnki.dlhyxb.2023-120 |
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| Expression characteristics of CgLOX4 gene in larval development of Pacific oyster (Crassostrea gigas) |
| HU Yue，LENG Jinyuan，SUN Jiejie*，WANG Lingling*，SONG Linsheng
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| Dalian Key Laboratory of Aquatic Animal Diseases Prevention and Control，Liaoning Key Laboratory of Marine Animal Immunology and Epidemic Prevention and Control，Dalian Ocean University，Dalian 116023，China |
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Abstract To analyze the expression characteristics of CgLOX4 during larval development, the full-length cDNA sequence of LOX4 was amplified from Pacific oyster (Crassostrea gigas) by using PCR, and the expression characteristics of CgLOX4 in different stages of larval development were further analyzed by qRT-PCR and whole-mount immunofluorescence experiments. It was found that the CgLOX4 gene had full-length cDNA sequence of 1 862 bp with an open reading frame of 834 bp encoding 277 amino acids, with the isoelectric point of 8.36. The amino acid sequence of CgLOX4 gene contained a conserved HOX domain, and CgLOX4 gene was expressed in all the tested tissues of adult oyster, with significantly higher expression level in the adductor muscle than that in hepatopancreas, gills, hemolymph, gonad and labial palps tissue (P<0.05). CgLOX4 was expressed in all the examined early embryos, with relatively high expression level in fertilized eggs, and four-cells. The whole-mount immunofluorescence assay revealed that the positive signals of LOX4 were mainly distributed in the shell field of trochophore, the visceral mass of D-shape larvae and the digestive gland of umbo larvae. The findings indicate that CgLOX4 gene is one of the transcription factors activated earlier in the early development process of the oyster, and may play an important role in regulating the formation of larval shell and digestive organ.
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Published: 13 March 2024
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LUO Xiaonian, DUAN Youjian, ZHANG Xiaojing, ALIDA·Xiamuxiding, LI Jiao, WU Chen, LI Wenkang, JI Chenyue, CHANG Jiantai. Artificial propagation and embryonic and larval development of Chinese hook snout carp Opsariichthys bidens[J]. Journal of Dalian Ocean University, 2022, 37(4): 550-557. |
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