Journal of Dalian Ocean University  2024, Vol. 39 Issue (1): 29-37 DOI: 10.16535/j.cnki.dlhyxb.2023-219 |
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| Effects of bacterium-alga synergism on aquatic environment, growth and immunoenzymatic activities in largemouth bass (Micropterus salmoides) culture |
| ZENG Tianyu，CHEN Xi，XU Huimin，MENG Shunlong*，JIN Yuqin，HU Gengdong，LI Dandan，QIU Liping，SONG Chao，FAN Limin，XU Pao
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| 1.College of Fisheries and Life Science，Shanghai Ocean University，Shanghai 201306，China；2.Laboratory of Quality &Safety Risk Assessment for Aquatic Products on Environmental Factors (Wuxi)，Ministry of Agriculture and Rural Affairs，Key Open Laboratory of Ecological Environment and Resources of Inland Fisheries，Chinese Academy of Fishery Sciences，Freshwater Fisheries Research Center，Chinese Academy of Fishery Sciences，Wuxi 214081，China；3.Wuxi Fisheries College，Nanjing Agricultural University，Wuxi 214081，China |
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Abstract In order to explore the effects of addition of green alga (Chlorella vulgaris), bacterium (Bacillus subtilis) and mixture of C.vulgaris and B.subtilis on aquatic environment, growth performance and related enzyme activities of largemouth bass (Micropterus salmoides), juvenile largemouth bass with body weight of (11.71±0.90)g was reared in a 1 000 L polyethylene barrel filled with 800 L water added with C.vulgaris (density of 1×109 cells/L at 6.25 mL/L)(alga group), B.subtilis (1×1011 CFU/g at 0.75 mg/L) (bacterium group), both C.vulgaris and B.subtilis (bacterium + alga mixture group), and blank (without any bacterium and alga, control group for days). The results showed that there were generally optimised physical and chemical indices in the water environment in the alga group and bacterium group during the experiment, compared with the blank group, with the relatively small differences in the total nitrogen, total phosphorus and dissolved oxygen contents in each experimental group, while there were considerably varied in the contents of ammonia nitrogen, nitrate nitrogen and nitrite nitrogen, especially in the water environment in the bacterium + alga mixed group, with the minimal ammonia nitrogen, nitrate nitrogen and nitrite nitrogen contents in water in most time points (P<0.05). At the end of the experiment, the largemouth bass in all experimental groups had increase in body weight, weight gain rate, and specific growth rate compared with the blank group, with significant differences in only the bacterium + alga mixture group compared with the blank group (P<0.05). The hepatic glutathione (GSH) content, and activities of catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and lysozyme (LZM) in general were shown to be significantly increased (P<0.05), and malondialdehyde (MDA) content to be significantly decreased (P<0.05), especially in the bacterium + alga mixture group compared with the blank group. To sum up, it can be seen that addition of either C.vulgaris or B.subtilis to the water environment can improve the water quality, promote the growth and hepatic antioxidant capacity and immunity in largemouth bass, and addition of both the green alga and bacterium at the same time is more effective.
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Published: 13 March 2024
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ZHAO Junhui, WANG Yang, WANG Yakun, WU Pengju, HUANG Na, CHEN Liping, LIU Qigen, HU Zhongjun. Phytoplankton community structure, functional groups and their relationship with environmental factors in Dongguan Ecological Park[J]. Journal of Dalian Ocean University, 2023, 38(6): 1020-1031. |
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