Journal of Dalian Ocean University  2009, Vol. 24 Issue (4): 300-304 DOI: 10.3969/j.issn.1000-9957.2009.04.003 |
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| Influence of some physical and chemical factors on the complement hemolytic activity in sea cucumber Apostichopus japonicus |
| ZHANG Feng, ZHANG Li
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| 1. School of Life Science and Technology, Dalian Fisheries University, Dalian 116023, China ;2. Key Laboratory of Marine Bio - resources Sustainable Utilization in Liaoning Provinc＇s University, Dalian Fisheries Univ. , Dalian 116023 ,China |
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Abstract The complement hemolytic activity was reported in the coelomic fluid of sea cucumber Apostichopus japonicus, a rate of （68.8 ± 13.5） U/mL. The ACHso assay revealed that the optimum conditions for the complement hemolytic activity in the coelomic fluid were as the following- pH 7.5,8 mmol/L Mg^2＋ EGTA - Mg - GVB buffer, at 20 ℃ and reaction period of 90 min. The haemolytic activity was affected by Mg^2＋ and inhibited by zymosan, methylamine, hydrazine and PMSF.
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Published: 30 December 2016
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