WANG Ya-yue1,2,XIN Yan-juan1,ZHANG Jin-you1,2,XUE Song1
1.Marine Bioproducts Engineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023,China;2.University of Chinese Academy of Sciences, Beijing 100049,China
Abstract: The effects of carbon source substrates DL-2-chloropropionic acid(DL-2-CPA), L-2-chloropropionic acid(L-2-CPA) and D-2-chloropropionic acid(D-2-CPA) on induction of 2-haloacid dehalogenases L-2-haloacid dehalogenase(L-DEX) and D-2-haloacid dehalogenase(D-DEX) were studied and culture process was optimized in marine bacteriumPseudomonasstutzeriDEH138A. The residual chloropropionic acid in the reaction system was quantified by HPLC. The L-2-CPA as sole carbon source was shown to specifically induce L-DEX production, with 84 times higher activity than D-DEX. The maximum activities of L-DEX (6.11×10-2U/mL media) and D-DEX (3.23×10-3U/mL media) were observed under culture condition of sole carbon source of L-2-CPA, 5.1 times and 2.3 times as the induction of DL-2-CPA. The findings indicate that L-2-CPA shows specificity for directional induction of L-DEX. For D-DEX, however, it is not explained by the mechanism of L-DEX which is induced by corresponding substrate, and the induction of D-DEX needs to be further studied.
2014, Vol. 29 
