Abstract: Filaments were induced the crusts of sea weed Grateloupia filicima peeled off from the substratum and incubated at water temperature of 10, 15 or 20 ℃. The most rapid formation and the maximal production of the filaments were observed at 20 ℃, while the best growth occurred at 15℃. The cells of the filament＇ s fragment adherent to the substratum gave rise to crusts. The optimal incubation temperature was 20 ℃ for the crusts production. From 50 um to 250 um, the shorter the fragment was, the more easily was the crusts produced. The crusts exhibited an optimal growth under 20 ℃ and the erect thalli were generated from them in their size of around 200 um. There was significant influence of temperature on the generation of erect thalli. The erect thalli had best growth at 20 ℃. The seedling incubation was conducted by chopped filaments into fragments, and germination was obtained in two months through indoor culture.
2007, Vol. 22 
