Abstract: Tissue profiles and expression of immunoglobulin M heavy chain mRNA (IgHM mRNA)in normal and bacterial challenging Siberian sturgeonAcipenserbaeriiwith body weight of (260.2±37.8) g were investigated by a primer designed based on Siberian sturgeon in GenBank，and by Sybrgreen real time PCR. The results showed that IgHM mRNA was expressed in abundance in spleen，a little amount of IgHM mRNA expression in liver and heart, and almost no IgHM mRNA expression in gill, hindgut, muscle, gonad and brain. The mortality was observed in the Siberian sturgeon with muscular injection of pathogenic bacteriumAeromonasveroniiwith density of 2.0×107CFU/mL at a dose of 0.1 mL/100 g body weight at water temperature of 22.6-24.5 ℃ 40 h after injection. Sixty-two h after injection, however, no mortality was found in the Siberian sturgeon with IgHM mRNA expression in spleen at a level as that in the fish in the control group. The Siberian sturgeon challenged for 93 h showed as higher 3.7 times IgHM mRNA expression in spleen as that in the fish in the control group, indicating that the challenged Siberian sturgeon had activated specific immune response.
2014, Vol. 29 
