Genetic relationship between Furong crucian carp（Furong carp♀×red crucian carp♂） and the original brood stocks
WANG Jin-long, LIU Zhen, LI Chuan-wu , WANG Dong-wu, ZENG Guo-qing, LU Shuang-qing
1. Institute of Fishery Sciences of Hunan Province, Changsha 410153, China; 2. Department of Biotechnology and Environment Science, Changsha Uni- versity, Changsha 410003, China ;3. Key Open Laboratory for Genetic Breeding of Aquatic Animals and Aquaculture Biology, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081 ,China
Abstract: Furong crucian carp（Furong carp♀×red crucian carp♂） is a hybrid that combined with remote hybridization between carp and crucian carp and close crossing among breeds of carp.In original brood stocks of Furong crucian carp（red crucian carp,Furong carp,scattered scale mirror carp and Xingguo red carp）,two red stocks were used.Previous research suggested that there was a heterosis when red brood stock was used in hybridization between carp and crucian carp.The present dealt with the genetic relationship between Furong crucian carp and the four original brood stocks by random amplified polymorphic DNA（RAPD） and microsatellite DNA methods,and the genetic effect of red stocks was discussed.In total 20 RAPD primers,it was found that 12 primers amplified the proper DNA bands in five populations of fish.The genetic identities of Furong crucian carp and red crucian carp were found to be 0.8757,Furong carp 0.7478,scattered scale mirror carp 0.7419 and Xingguo red carp 0.7449,and the genetic distances were 0.1327,0.2906,0.2985,0.2944,respectively,based on the RAPD bands.In total 29 microsatellite primers,there were 13 primers that amplified the proper DNA bands in five populations of fish.The microsatellite bands analysis showed that the genetic identities of Furong crucian carp and red crucian carp were found to be 0.8717,Furong carp 0.7434,scattered mirror carp 0.6680 and Xingguo red carp 0.7552 and,the genetic distances were found to be 0.1374,0.2965,0.4034,and 0.2808,respectively.It can be conclusion that the genetic structure of Furong crucian carp was biased to the male（red crucian carp） and grandfather（Xingguo red carp）,indicating consistent with the theory that there is a close relation ship between genetic basis of the red broodstock and heterosis.
王金龙, 刘臻, 李传武, 王冬武, 曾国清, 鲁双庆. 芙蓉鲫(芙蓉鲤♀×红鲫♂)及其原始亲本间的遗传关系[J]. 大连海洋大学学报, 2010, 25(3): 197-202.
WANG Jin-long, LIU Zhen, LI Chuan-wu , WANG Dong-wu, ZENG Guo-qing, LU Shuang-qing. Genetic relationship between Furong crucian carp（Furong carp♀×red crucian carp♂） and the original brood stocks. Journal of Dalian Ocean University, 2010, 25(3): 197-202.
2010, Vol. 25 
