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大连海洋大学学报  2007, Vol. 22 Issue (1): 49-52    DOI: 10.3969/j.issn.1000-9957.2007.01.011
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扇贝加工废弃物自溶技术的研究
郑丽,汪秋宽,谢智芬,汪涛
大连水产学院,辽宁省水产品加工及综合利用重点开放实验室,辽宁,大连,
The rapid autolytic technology of scallop processing wastes
ZHENG Li, WANG Qiu-kuan, XIE Zhi-fen, WANG Tao 
The Key and Open Laboratory of Fishery Product Processing and Utilization of Liaoning Province, Dalian Fisheries Univ. , Dalian 116023, China
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摘要 对扇贝加工废弃物的自溶水解技术进行了研究。结果表明:用紫外线照射扇贝加工废弃物后能够激活自溶酶,当照射40min时,自溶水解液中游离氨基酸态氮含量最高(3.389g/L);pH对自溶水解影响较大,pH为7.0时水解效果最佳;采用40~65℃(5℃/h)的梯度升温自溶水解4h或60℃恒温下自溶水解3h,其水解效果最佳;元机离子Na^+、Ca^2+对自溶酶具有激活作用,当Na^+的浓度为0.07mol/L、Ca^2+的浓度为0.03mol/L时,自溶水解效果最佳,而Zn^2+对自溶酶的影响很小;扇贝加工废弃物在最佳条件下自溶水解3h后(60℃恒温),其水解液中氨基酸态氮的含量为4.006g/L。用SephadexC-15葡聚糖凝胶柱层析法对扇贝加工废弃物的自溶水解产物进行分析,结果显示,水解液中蛋白肽相对分子质量集中分布在784.25左右,说明白溶水解产物多为小分子的蛋白肽,可进一步对其开发利用,制备海鲜调味料等。
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郑丽
汪秋宽
谢智芬
汪涛
关键词:  扇贝加工废弃物  自溶  分子量分布    
Abstract: A fundamental pattern was proposed for rapid autolysis of scallop disposal from processing by a series of experiments on factors of UV radiation, mineral ions, pH value and temperature. It was found that there was the maximal amino acid nitrogen concentration (3. 389 g/L) in the scallop disposal solution under 40 min UV radiation ; good autolysis was observed at pH value of 7.0; the rate of autolysis was accelerated at 60℃ or a gradient temperature of 5℃/h from 40℃ 5 to 65 ℃ ; The rate of hydrolysis for scallop disposal increased at Na ion of 0.07 mol/L and Ca ion of 0.03 mol/L while there was no significant effect of Zn ion concentration on the rate of autolysis. The amino acid nitrogen of 4. 006 g/L was found in the hydrolysate under the optimal autolytic conditions for 3 hr. The analysis of molecular distribution of the autolyzed hydrolysates by SephadexG - 15 resin showed that the molecules primarily consisted of small molecular peptides with the molecular weight of about 784.25 Da, which can be used as essential part of the nutritious and delicate fishery seasonings.
Key words:  scallop waste disposal from processing    autolysis    molecular weight distribution
                    发布日期:  2016-12-30      期的出版日期:  2007-02-21
中图分类号:  TS254.9  
引用本文:    
郑丽, 汪秋宽, 谢智芬, 汪涛. 扇贝加工废弃物自溶技术的研究[J]. 大连海洋大学学报, 2007, 22(1): 49-52.
ZHENG Li, WANG Qiu-kuan, XIE Zhi-fen, WANG Tao . The rapid autolytic technology of scallop processing wastes. Journal of Dalian Ocean University, 2007, 22(1): 49-52.
链接本文:  
https://xuebao.dlou.edu.cn/CN/10.3969/j.issn.1000-9957.2007.01.011  或          https://xuebao.dlou.edu.cn/CN/Y2007/V22/I1/49
[1] 郑丽, 汪秋宽. 扇贝加工废弃物蛋白酶解及其酶解产物分子量分布的研究[J]. 大连海洋大学学报, 2006, 21(2): 149-152.
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