Abstract: By means of microinjection, fusion gene consisting of MT operator of common carp and growth hormone gene of salmon was injected in fertilized eggs released by rainbow trout, common carp and crucian carp respectively. Hatching rates averaged 6.4%, 34.0%, 35.7% respectively after injecting foreign gene. According to survival rates calculated by hatching fries, the difference and influential factors on hatching rate of these fishes were studied and analysed . There was no distinctive difference between common carp and crucian carp in hatching rate, whereas remarked differences were detected by comparing the former fishes with the rainbow trout using t test. The results indicate microinjection method can make a great difference on the transgenic effects of fertilized eggs in these fishes.
闫学春, 孙效文, 梁利群, 曹顶臣. 几种鱼类的受精卵注射外源基因后孵化率的比较研究[J]. 大连海洋大学学报, 2003, 18(2): 145-148.
YAN Xue-chun, SUN Xiao-wen, LIANG Li-qun, CAO Ding-cheng. Comparative studies on hatching rate of fertilized eggs injected foreign gene in several fishes. Journal of Dalian Ocean University, 2003, 18(2): 145-148.
2003, Vol. 18 
