四溴联苯醚慢性胁迫对黄盖鲽幼鱼鳃的毒性效应

doi:10.16535/j.cnki.dlhyxb.2024-035

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大连海洋大学学报 ›› 2024, Vol. 39 ›› Issue (5) : 746-753. DOI: 10.16535/j.cnki.dlhyxb.2024-035

四溴联苯醚慢性胁迫对黄盖鲽幼鱼鳃的毒性效应

崔丹丹, 孙鹏飞, 郭亚枫, 曹胜男, 韩建, 顾知之, 王伟*

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Toxic effects of tetrabromodiphenyl ether on gills of marbled flounder (Pseudopleuronectes yokohamae) exposed to chronic stress

CUI Dandan，SUN Pengfei，GOU Yafeng，CAO Shengnan，HAN Jian，GU Zhizhi，WANG Wei*

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摘要

为研究四溴联苯醚(BDE-47)对黄盖鲽(Pseudopleuronectes yokohamae)幼鱼(体质量为1.31 g±0.03 g)鳃组织的毒性效应，采用半静态水质接触染毒法，通过组织切片观察了BDE-47胁迫下(0.25、0.5、2 mg/L)黄盖鲽鳃组织的形态学变化，并利用mRNA测序和miRNA测序联合分析黄盖鲽幼鱼鳃组织对BDE-47胁迫的分子机制。结果表明，在BDE-47胁迫下，黄盖鲽鳃小片上皮细胞出现肿胀、脱落及增生修复现象，黏液细胞明显增多，且随着BDE-47浓度升高呈递增趋势；转录组数据显示，ErbB信号通路基因表达量变化可能引起鳃小片上皮细胞的增生和修复；miRNA测序分析显示，BDE-47暴露导致的鳃小片上皮细胞脱落可能与MAPK及Ras信号通路有关；在mRNA和miRNA联合分析中，主要关注了硫辛酸代谢通路和Toll样受体信号通路，推测鳃组织miRNAs通过调节硫辛酸代谢和Toll样受体信号通路基因mRNA的稳定性和翻译，影响水体中BDE-47对黄盖鲽幼鱼鳃的毒性效应。研究表明，不同浓度BDE-47慢性胁迫会对黄盖鲽鳃组织结构造成不同程度的损伤，通过转录组联合分析发现，差异基因主要富集在炎症、凋亡和免疫调节等相关信号通路中。

Abstract

In order to investigate the toxic chronic effects of tetrabromodiphenyl ether (BDE-47) on juvenile marbled flounder (Pseudopleuronectes yokohamae), the juvenile marbled flounder with body weight of 1.31 g±0.03 g were kept in a culture tank containing 40 L of water and exposed to blank control (CK), solvent control group (DMSO), 0.25 (LC), 0.5 (MC), and 2.0 mg/L BDE-47 (HC) at water temperature of (18.4 ±0.85) ℃ by semi-static water contact exposure method, and the morphological changes of gill tissue were histologically observed in the juvenile marbled flounder exposed to BDE-47 stress. The molecular mechanism of gill tissue under BDE-47 stress was analyzed by miRNA sequencing and mRNA sequencing. The results showed that swelling, shedding and hyperplasia repair, and significant increase in mucus cells were observed in the epithelial cells of gill lamellae of the marbled flounder exposed to BDE-47 stress, more severe with the elevated BDE-47 concentration. Transcriptome data showed that changes in the expression of ErbB signaling pathway genes caused the proliferation and repair of gill lamellae epithelial cells. miRNA sequencing analysis revealed that the shedding of gill lamellae epithelial cells caused by BDE-47 exposure was involved in MAPK and Ras signaling pathways. The lipoic acid metabolism pathway and Toll-like receptor signaling pathway were primarily in the combined analysis of mRNA and miRNA, speculating that miRNAs in gill tissue affected the toxic effect of BDE-47 on the gills of marbled flounder in water by regulating the stability and translation of lipoic acid metabolism and Toll-like receptor signaling pathway gene mRNA. The findings indicate that chronic stress with different concentrations of BDE-47 can cause different degrees of damage to the gill tissue structure of marbled flounder.

导出引用

崔丹丹, 孙鹏飞, 郭亚枫, 曹胜男, 韩建, 顾知之, 王伟. 四溴联苯醚慢性胁迫对黄盖鲽幼鱼鳃的毒性效应[J]. 大连海洋大学学报, 2024, 39(5): 746-753 https://doi.org/10.16535/j.cnki.dlhyxb.2024-035

CUI Dandan, SUN Pengfei, GOU Yafeng, CAO Shengnan, HAN Jian, GU Zhizhi, WANG Wei. Toxic effects of tetrabromodiphenyl ether on gills of marbled flounder (Pseudopleuronectes yokohamae) exposed to chronic stress[J]. Journal of Dalian Fisheries University, 2024, 39(5): 746-753 https://doi.org/10.16535/j.cnki.dlhyxb.2024-035

中图分类号： S 917.4