附着型硅藻生物膜构建及光照强度对其氨氮吸收效果的影响

doi:10.16535/j.cnki.dlhyxb.2024-007

PDF(8249 KB)

大连海洋大学学报 ›› 2024, Vol. 39 ›› Issue (5) : 829-837. DOI: 10.16535/j.cnki.dlhyxb.2024-007

附着型硅藻生物膜构建及光照强度对其氨氮吸收效果的影响

刘楚瑶，王鑫，陈丽红，张洪霞，于贞，李蕊，邢荣莲*

作者信息 +

Construction of attached diatom biofilm and effect of light intensity on ammonia nitrogen absorption by the biofilm

LIU Chuyao，WANG Xin，CHEN Lihong，ZHANG Hongxia，YU Zhen，LI Rui，XING Ronglian*

Author information +

文章历史 +

摘要

为解决藻膜中藻生物量不理想，光照以强光为主等问题，对附着型硅藻——卵形藻(Cocconei sp.)生物膜基质进行筛选，构建出卵形藻帆布网生物膜，研究了光照强度对该藻膜氨氮的吸收效果和藻体生长情况。结果表明：粗糙的麻布网和帆布网基质更有利于卵形藻的附着生长(P<0.05)，其中，帆布网基质上卵形藻细胞密度最高可达(1.80±0.52)×106 ind./mL；在不同光照条件下，卵形藻对氨氮吸收效果有显著性差异(P<0.05)，在96 h时，当光照强度达到54 μmol/(m2·s)，氨氮浓度为20 mmol/L时，生物膜上卵形藻的吸收率可达98.5%；氨氮浓度为60 mmol/L时，吸收率可达86.7%，此时卵形藻采收细胞密度可达(2.90±0.12)×106 ind./mL，类胡萝卜素含量为(0.520±0.013)mg/mL，可作为水生生物优质饵料。研究表明，帆布网基质更有利于卵形藻的附着，当光照强度为[18～54 μmol/(m2·s)]时，卵形藻生物膜具有良好的氨氮吸收能力。

Abstract

In order to solve the problems including unideal biomass of algae in the algal biofilm and constrain of strong light, the biofilm matrix of attached diatom (Cocconei sp.) was screened, and the constructed Cocconei canvas mesh biofilm with the attached diatom at density of 1×105 ind/mL was cultured in F/2 medium at light intensity of 0, 1, 8, 18, 54, 90 and 126 μmol/(m2·s) at temperature of (20±2) °C and the light period of 12 L∶12 D for 9 days , and then, the effect of illumination intensity on the absorption of ammonium nitrogen at concentration of 0, 20, 60 and 100 mmol·L-1 and the growth of the algae subsequently in this biofilm. The results showed that coarse linen and canvas mesh were more conducive to the apposition growth of Cocconei sp. (P<0.05), with the maximal biomass of (1.80±0.52)×106 ind./mL in canvas mesh. The significant variation in absorption effect of Cocconei sp. on ammonium nitrogen was found under different illumination conditions (P<0.05), with absorption rate of 98.5% by Cocconei sp. at ammonium nitrogen concentration of 20 mmol/L, at illumination intensity 54 μmol/(m2· s) in 96 h. Meanwhile, the absorption rate of 86.7% was observed at ammonium nitrogen concentration of 60 mmol/L, with the Cocconei sp. harvested biomass of (2.90±0.12)×106 ind./mL, and the carotenoid content of (0.520±0.013) mg/mL, which could be used as high quality forage for aquatic animals. The results showed that the substrate was more conducive to the adhesion of Cocconei sp., and the alga biofilm grew in a wide range of light, and had a good ammonia nitrogen absorption capacity of up to 98.3% at light intensity of 18-54 μmol/(m2·s). Therefore, the alga biofilm obtained in the present experiment has good application value.

导出引用

刘楚瑶, 王鑫, 陈丽红, 张洪霞, 于贞, 李蕊, 邢荣莲. 附着型硅藻生物膜构建及光照强度对其氨氮吸收效果的影响[J]. 大连海洋大学学报, 2024, 39(5): 829-837 https://doi.org/10.16535/j.cnki.dlhyxb.2024-007

LIU Chuyao, WANG Xin, CHEN Lihong, ZHANG Hongxia, YU Zhen, LI Rui, XING Ronglian. Construction of attached diatom biofilm and effect of light intensity on ammonia nitrogen absorption by the biofilm[J]. Journal of Dalian Fisheries University, 2024, 39(5): 829-837 https://doi.org/10.16535/j.cnki.dlhyxb.2024-007

中图分类号： S 968.4 X 505