棘皮动物弧菌诱导中间球海胆免疫致敏现象分析

doi:10.16535/j.cnki.dlhyxb.2023-196

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大连海洋大学学报 ›› 2024, Vol. 39 ›› Issue (2) : 266-274. DOI: 10.16535/j.cnki.dlhyxb.2023-196

棘皮动物弧菌诱导中间球海胆免疫致敏现象分析

殷维骏, 刘岩松, 田文卓, 欧凡江, 刘雷, 丁君, 张伟杰*, 常亚青*

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Analysis of immune priming phenomenon in sea urchin (Strongylocentrotus intermedius) induced by Vibrio echinoideorum

YIN Weijun，LIU Yansong，TIAN Wenzhuo，OU Fanjiang，LIU Lei，DING Jun，ZHANG Weijie*，CHANG Yaqing*

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摘要

为探究中间球海胆(Strongylocentrotus intermedius)是否存在免疫致敏现象，使用棘皮动物弧菌(Vibrio echinoideorum)对中间球海胆进行重复感染(首次、二次菌浓度分别为103、104 CFU/mL)，检测并比较了两次注射感染后不同时间点中间球海胆体腔细胞密度、吞噬能力、酸性磷酸酶(ACP)活力、活性氧(ROS)含量、总抗氧化能力(T-AOC)等相关免疫指标的变化，以及二次感染后免疫致敏组和对照组间成活率的差异。结果表明：与首次感染相比，二次感染时诱导致敏组的体腔细胞总密度、吞噬细胞密度、ACP、ROS和T-AOC等均出现显著上升(P<0.05)，且响应时间明显缩短，达到峰值的时间分别提前了36、36、84、12、36 h，且各指标的峰值均显著高于首次感染(P<0.05)；二次感染后，诱导致敏组在6 h时的吞噬率(45.41%±6.39%)显著高于对照组(33.17%±1.94%)；经过棘皮动物弧菌低浓度诱导后，诱导致敏组存活率(43.33%±10.00%)显著高于未经诱导的阳性对照组(7.78%±10.71%)(P<0.05)。研究表明，低浓度棘皮动物弧菌能诱导中间球海胆产生免疫致敏现象，此结果可为中间球海胆的疾病防控提供新思路。

Abstract

To investigate whether immune priming exists in sea urchin (Strongylocentrotus intermedius), twice infection with Vibrio echinoideorum was conducted. Differences in immune parameters such as phagocytic amoebocyte density, red spherule cell density, phagocytic rate, phagocytic index, acid phosphatase activity (ACP), reactive oxygen species (ROS) content, and total antioxidant capacity (T-AOC) between the two time infections were compared. Difference in survival rate post challenge between immune priming group and control group were also compared. The results showed that the total coelomocytes cell density, phagocyte density, ACP, ROS and T-AOC in the induced immune priming group increased significantly after the second infections (P<0.05), and the response time to the maximum was 36, 36, 84, 12 and 36 h earlier respectively, and the peak values were significantly higher than those in the first infection (P<0.05). The phagocytosis rate was significantly higher in the induced priming group (45.41%±6.39%) than that in the control group (33.17%±1.94%) at 6 h post infection. The survival rate was significantly higher in the induced immune priming group (43.33%±10.00%) than that in the uninduced positive control group (7.78%±10.71%) (P<0.05). The findings indicate that low concentrations of V.echinoideorum can induce immune priming in the sea urchin, which provides new ideas for prevention and control of diseases in sea urchin.

导出引用

殷维骏, 刘岩松, 田文卓, 欧凡江, 刘雷, 丁君, 张伟杰, 常亚青. 棘皮动物弧菌诱导中间球海胆免疫致敏现象分析[J]. 大连海洋大学学报, 2024, 39(2): 266-274 https://doi.org/10.16535/j.cnki.dlhyxb.2023-196

YIN Weijun, LIU Yansong, TIAN Wenzhuo, OU Fanjiang, LIU Lei, DING Jun, ZHANG Weijie, CHANG Yaqing. Analysis of immune priming phenomenon in sea urchin (Strongylocentrotus intermedius) induced by Vibrio echinoideorum[J]. Journal of Dalian Fisheries University, 2024, 39(2): 266-274 https://doi.org/10.16535/j.cnki.dlhyxb.2023-196

中图分类号： S 947.9