Structural composition and hypolipidemic effect of fucoidan from Sargassum horneri
LIU Wen，CHE Xinyi，MA Zhichao，LIU Shu，REN Dandan，WANG Qiukuan，HE Yunhai*，WANG Junfeng
1. National Research and Development Branch Center For Seaweed Processing，Key Laboratory of Aquatic Product Processing and Utilization of Liaoning Province，College of Food Science and Engineering，Dalian Ocean University，Dalian 116023，China；2.Collaborative Innovation Center of Seafood Deep Processing(Dalian Polytechnic University)，Dalian 116034，China
Abstract: In order to explore the structural composition and hypolipidemic effect of fucoidan sulfate from, Sargassumhorneri, fucoidan (SF) was extracted from the Sargassumhorneri by enzymatic hydrolysis, in which the purity of polysaccharides was determined, and the monosaccharide composition and functional composition of SF were analyzed. The organ coefficients, serum lipid indices and antioxidant activity indices were detected in KM mice with high-fat model via gastric gavage of SF at dose of 100, 300, and 500 mg/kg 28 days after gastric gavage. The results showed that there were extraction rates of 3.68% for fucoidan, 42.63% for polysaccharides content, and 13.35% for sulfate content by composite enzymatic hydrolysis. Infrared spectroscopy combined with monosaccharide composition analysis revealed that SF was composed of fucose (39.83%) and galactose (28.37%) as the main monosaccharides β-pyranose cyclic sulfate polysaccharide. Animal test showed that the increase in weight and organ coefficients caused by high fat was shown to be significantly declined, the levels of total cholesterol (TC), triglycerides (TG), low-density lipoprotein (LDL-C) to be significantly reduced (P<0.05), and to be enhanced in the level of high-density lipoprotein (HDL-C) in serum of the mice with different gavage doses of SF (P<0.05) compared with the model group. Malondialdehyde (MDA) content in liver was significantly reduced (P<0.05), glutathione peroxidase (GSH-Px) activity and nitric oxide (NO) level were significantly reduced (P<0.05). SF led to significantly reduce atherosclerotic index (AI) value and to be very low density lipoprotein (VLDL-C) concentration (P<0.05). There was better effect of reducing blood lipid in the three SF dosage groups than that in the drug (P<0.05), with the best blood lipid lowering effect in the high dosage group (500 mg/kg). The findings indicate that fucoidan sulfate from Sargassumhorneri can effectively regulate abnormal lipid metabolism, prevent atherosclerosis, and good hypolipidemic activity.
刘雯, 车心怡, 马志超, 刘舒, 任丹丹, 汪秋宽, 何云海, 王俊峰. 铜藻岩藻聚糖硫酸酯的结构组成及降血脂作用[J]. 大连海洋大学学报, 2023, 38(2): 323-330.
LIU Wen, CHE Xinyi, MA Zhichao, LIU Shu, REN Dandan, WANG Qiukuan, HE Yunhai, WANG Junfeng. Structural composition and hypolipidemic effect of fucoidan from Sargassum horneri. Journal of Dalian Ocean University, 2023, 38(2): 323-330.
2023, Vol. 38 
