1.College of Marine Science and Engineering，Nanjing Normal University，Nanjing 210023，China；2.Jiangsu Provincial Fine Breeds of Meretrix，Jiangsu Institute of Marine Fisheries，Nantong 226007，China；3.Tongzhou Fishery Technical Guidance Station，Nantong 226300，China
Abstract: In order to explore the changes in transcriptome of jackknife clam (Solengrandis) after siphon autotomy, the transcriptome of jackknife clam with shell length of 8-10 cm before (control group) and 7 h, 7 d and 20 d after siphon autotomy (selection group) were analyzed by second-generation sequencing technology. The results showed that 103 909 Unigenes were obtained from the raw data after splicing and annotation, in which 30 027 Unigenes were annotated, with an annotation rate of 28.90%. The gene ontology functional enrichment results of three groups of differentially expressed genes showed that the calcium-binding items were the most enriched at 7 hours after autotomy, and the chitin-binding items were the most enriched at 7 days and 20 days after autotomy. The results of KEGG enrichment pathway showed that the jackknife clam had significantly enriched pathways only 7 days after autotomy, including amino sugar and nucleotide sugar metabolism, nitrogen metabolism, focal adhesion, lysosome and MAPK signal pathway. The time trend analysis of all differentially expressed genes in three groups showed that the top three profiles of gene enrichment were Profile5, Profile17 and Profile12. The gene ontology function annotation results of the three patterns showed that 529 genes in Profile5 were significantly enriched to ion-binding items, accounting for 53.77%, and the items with the largest number of genes enriched were receptor activity in Profile17 and chitin metabolic activity in Profile12. The RT-qPCR verification of six differentially expressed genes proved that the transcriptome sequencing results were reliable, and that preliminarily speculated various signal molecules represented by calcium ions played an important role in the process of transmitting damaged signals in the early stage of siphon regeneration (7 hours after autotomy), meanwhile the activity of various receptors was enhanced in response to the siphon regeneration process. With the progress of regeneration, the processes of substance metabolism and biosynthesis were greatly increased to meet the needs of tissue regeneration.
2023, Vol. 38 
