Selective breeding and growth performance evaluation of the high-light-tolerant Betaphycus gelatinus mutant strain XY-01
LI Xinyu，ZHU Jun，LI Qingnan，ZOU Xiaoxiao，BAO Shixiang*，AN Xinlong*
1.Ocean College，Agriculture University of Hebei，Qinhuangdao 066003，China；2.Hainan Institute for Tropical Agricultural Resources，Institute of Tropical Bioscience and Biotechnology，CATAS &Key Laboratory of Biology and Genetic Resources of Tropical Crops of Hainan Province，Haikou 571101，China；3.Zhanjiang Experimental Station，CATAS，Zhanjiang 524013，China；4.Hainan Provincial Key Laboratory for Functional Components Research and Utilization of Marine Bio-resources，CATAS，Haikou 571101，China
Abstract: In order to improve the current situation of yield and quality reduction of Betaphycusgelatinus induced by high light stress, potential resistant strains were screened, isolated, and purified. A stable high-light-tolerant mutant strain, B.gelatinus XY-01, was obtained through subculture, and the growth performance of this mutant strain was evaluated subsequently. Compared to the wild-type strain (WT), the mutant strain XY-01 was green in color, had plump and stretched branches, and exhibited a less obvious high light stress phenotype. The contents of chlorophyll a, carotenoids and phycocyanin in XY-01 were all higher than those in WT at 26 ℃ with photosynthetic photon flux density of 60 μmol/(m2·s), while the content of phycoerythrin was much lower than that of WT. The optimum growth photosynthetic photon flux density of B.gelatinus XY-01 was 250 μmol/(m2·s). Under this condition, the maximum photochemical conversion efficiency of PSⅡ complex of XY-01 and WT were 0.635±0.019 and 0.484±0.055, respectively, with significant difference. The weight gain rates of XY-01 and WT were 57.00%±3.50% and 39.00%±4.28%, respectively (P<0.05). The specific growth rates were (1.61±0.09)%/d and (1.18±0.06)%/d, respectively (P<0.05). Marine culture experiments showed that the specific growth rate of XY-01 was (3.63±0.08)%/d from April to May, whereas, the specific growth rate of WT was only (2.63±0.37)%/d. In addition, the carrageenan content of XY-01 was not significantly different from that of WT (P>0.05). In this study, a stable, high-light-tolerant XY-01 was obtained for the first time through high-light selective breeding, which provides scientific reference for the development of germplasm resources of high-light-resistant B.gelatinus.
李欣宇, 朱军, 李青楠, 邹潇潇, 鲍时翔, 安鑫龙. 耐高光琼枝突变体XY-01的选育及生产性能评价[J]. 大连海洋大学学报, 2023, 38(3): 438-444.
LI Xinyu, ZHU Jun, LI Qingnan, ZOU Xiaoxiao, BAO Shixiang, AN Xinlong. Selective breeding and growth performance evaluation of the high-light-tolerant Betaphycus gelatinus mutant strain XY-01. Journal of Dalian Ocean University, 2023, 38(3): 438-444.
2023, Vol. 38 
