Embryonic and larval development and artificial breeding of sea urchin (Temnopleurus hardwickii)
HONG Jibiao，DING Shengqi，CHANG Yaqing，YIN Donghong，WANG Xubo，SONG Jian*
Key Laboratory of Mariculture &Stock Enhancement in North China’s Sea，Ministry of Agriculture and Rural Affairs，College of Fisheries and Life Science，Dalian Ocean University，Dalian 116023，China
Abstract: In order to realize the artificial breeding of sea urchin (Temnopleurushardwickii), induced to spawn, fertilization, incubation, larva rearing and adult cultivation of the sea urchin were carried out in a laboratory, then the developmental process of the sea urchin from fertilized egg to juvenile was observed under a microscope, and the size of fertilized egg, embryo and larvae was measured. The results showed that the sea urchin was successfully induced to spawn by injection of KCl solution, with fertilization rate of 93.6%±2.1%. The process of embryonic and larval development of the sea urchin was divided into several stages, including fertilized egg, cleavage stage, blastula stage, gastrula stage, prism larva, two armed larva, four armed larva, six armed larva, eight armed larva and juvenile. The fertilized eggs began to cleavage after 50 min, entered blastula stage after 5 h, gastrula stage after 10 h, prism larva stage after 18 h, two armed larva stage after 23 h, four armed larva stage after 1 d 16 h, six armed larva stage after 7 d 2 h, eight armed larva stage after 10 d 16 h, and metamorphosis into juvenile after 35 days at water temperature from 23 ℃ to 24 ℃. The sea urchin developed faster than other species of sea urchin before four armed larva and more slowly after from armed larva. The findings indicate that under condition of water temperature from 23 ℃ to 24 ℃ and salinity from 29 to 32, the ribed sea urchin can achieve artificial seeding cultivation and artificial reproduction indoors.
2023, Vol. 38 
