1.Fisheries College，Zhejiang Ocean University，Zhoushan 316022，China；2.Key Laboratory of Maricultural Organism Disease Control，Ministry of Agriculture and Rural Affairs，Qingdao Key Laboratory of Mariculture Epidemiology and Biosecurity，Yellow Sea Fisheries Research Institute，Chinese Academy of Fishery Sciences，Qingdao 266071，China；3.Hainan Zhongzheng Aquatic Science and Technology Company Limited，Dongfang 572632，China
Abstract: In order to explore the microbial control technology of bacterial pathogens of shrimp, an antibacterial test was performed by Oxford cup method on a strain of bacteria numbered 2021041402-14 (referred to as 02HN) isolated from shrimp nursery pond. The bacteriostatic effect and minimum inhibitory concentration of strain 02HN against six shrimp pathogens were analyzed, including Vibrioowensii, V.harveyi, V.campbellii, V.alginolyticus, V.parahaemolyticus and Photobacteriumdamselae. Meanwhile the strain 02HN was identified by comparison of 16S rRNA gene, gyrB-rpoD tandem gene sequence and physiological biochemical indices. Also the effect of this bacterium on Vibrio density was evaluated in Pacific white leg shrimp (Litopenaeusvannamei) fed diets containing the strain 02HN at concentrations of 0 (control group), 1×105 and 1×107 cells/g diet. The results showed that strain 02HN had obvious bacteriostatic effects on V.owensii, V.harveyi, V.campbellii, V.alginolyticus, V.parahaemolyticus and Photobacteriumdamselae with the minimal inhibitory concentrations at 2.32×104, 2.32×103, 2.32×106, 2.32×106, 2.32×103 and 2.32×106 CFU/mL respectively. Challenge test at concentration of 1×107 CFU/mL showed that there was no biological toxicity of strain 02HN to the Pacific white leg shrimp. Molecular biological identification results revealed that strain 02HN was a strain of Pseudodotalteromonas sp. closing to P.piscicida and P.flavipulchra in taxonomic status. There was no significant effect of strain 02HN content of 1×105 cells/g and 1×107 cells/g diet on survival and growth of the shrimp fed the diets containing strain 02HN, but significant decrease in the density of Vibrio in hepatopancreas. It can be seen that strain 02HN has the potential for development and application in shrimp farming. The findings provide biological materials and technical support for the biological control of bacterial pathogens of shrimp.
朱娜, 王秀华, 张红芳, 李婷, 王平, 张雪梅. 一株虾源假交替单胞菌（Pseudoalteromonas sp.）的分离鉴定及口服后对对虾体内弧菌的影响[J]. 大连海洋大学学报, 2023, 38(2): 223-232.
ZHU Na, WANG Xiuhua, ZHANG Hongfang, LI Ting, WANG Ping, ZHANG Xuemei. Isolation, identification and effects of a strain of Pseudoalteromonas sp. on Vibrio sp. in shrimp by oral administration. Journal of Dalian Ocean University, 2023, 38(2): 223-232.
2023, Vol. 38 
