Spatial differences in water microorganisms in middle and lower reaches of the Huaihe River in spring 2019
PENG Fei, ZHOU Yanfeng*, WANG Chenhe, ZHANG Xizhao, LUO Yuting, TANG Xuemei, ZHOU Yifan, WANG Dongwei
1.College of Wuxi Fisheries, Nanjing Agricultural University, Wuxi 214081, China; 2.Key Laboratory of Freshwater Fisheries and Utilization of Germplasm Resources, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China
Abstract: In order to understand the basic composition of microorganisms in the middle and lower reaches of the Huaihe River (Anhui Province-Jiangsu Province section), the composition and characteristics of microbial community structure were identified and analyzed by high-throughput sequencing technique and associated with the physical and chemical factors of the water body in 9 sections of four habitat types: tributaries, rivers, lakes (Hongze Lake) and estuaries in the middle and lower reaches of the Huaihe River (Anhui-Jiangsu section)in March 5 to 12, 2019 (dry season). The results showed that the microbial composition and richness of habitats were differentiated to a certain extent under different taxa, with gradual increase in the microbial diversity from tributaries to river channels, and the descending order of species richness in the different habitats as estuarine>Hongze Lake>tributaries>river courses. At the phylum level, Actinobacteria, Proteobacteria and Bacteroidetes were shown to be the common dominant groups at all sites. At the genera level, there were the maximal abundance of Flavobacterium, Rhodoferax and Limnohabitans at each site. Two endemic genera of Zymomonas and Methylibium were observed in river group, while HTCC, Arcobacter and Prosthecobacter were found to be unique in the other three habitat groups. The species with high richness at each sampling site mostly point to the water quality index with high nitrogen content, which converges with the results of water quality analysis, reflecting the basic situation of nitrogen pollution in various habitats. The horizontal dominant genera and Canonical correlation analysis (CCA) revealed that CODMn, total phosphorus (TP), turbidity (TUR), pH, transparency (SD) and were the main environmental factors affecting the composition of microorganisms in each site, combined with the correlation analysis and CCA between environmental factors and Alpha diversity. It was found that the middle and lower reaches of water can be divided into four different habitat types according to the difference in microbial community composition, suggesting that water microbes can be used as one of the indicators to reflect the water quality of the middle and lower reaches of the Huaihe River.
彭飞, 周彦锋, 王晨赫, 张希昭, 罗宇婷, 唐雪梅, 周依帆, 王东伟. 2019年春季淮河中下游水体微生物的空间异质性[J]. 大连海洋大学学报, 2022, 37(5): 830-840.
PENG Fei, ZHOU Yanfeng, WANG Chenhe, ZHANG Xizhao, LUO Yuting, TANG Xuemei, ZHOU Yifan, WANG Dongwei. Spatial differences in water microorganisms in middle and lower reaches of the Huaihe River in spring 2019. Journal of Dalian Ocean University, 2022, 37(5): 830-840.
2022, Vol. 37 
