Abstract: In order to explore enhancement effect of bleeding treatment on freshness-locked effect, the healthy living rock greenling Hexagrammosotakii were bleeding for 10 min by spinal cord cut and then kept in ice water cooling (well-bled, WB), and in conventional water cooling immediately after the spinal cord cut (poor-bled, PB) and the healthy living rock greenling was not bled as suffocation group (none-bled, NB). The two freshness locked groups were divided into two parts, one part was stored with ice for the observation of the characteristics of post mortem rigor mortis process, and the other part was placed in a 4 ℃ environment for cold storage stability analysis. The fish quality was evaluated by monitoring the biochemical indicators including contents of ATP and glycogen and rigor mortis process, combined with sensory analysis. Results showed that addition of room temperature water bleeding treatment before the fish body cooled led to significantly improve the bleeding effect: there was significantly higher average blood output of the enhanced freshness locked in group WB (8.6 g/ind.±3.1 g/ind.) than that in the conventional freshness-locked group PB (5.7 g/ind.±2.3 g/ind.)(P<0.05), enhanced bleeding showing superior cold storage stability. The various stages of the process of post-mortem rigor mortis were significantly delayed by enhanced bleeding effect can. During ice storage, the initial time of rigor mortis was about 4 hours later in the group WB than that in the group PB, and the time of rigor off was also about 6 hours later in the group WB. There was better preservation effect on ATP in the group WB, with ATP content of 1.20 μmol/g in the group WB, and only 0.58 μmol/g in the group PB 12 hours after chilled storage. Enhanced bleeding treatment resulted in significant improvement of the sensory quality of fish. During chilled storage, the abdominal muscles in the group WB showed bright silvery white in color, and the abdominal muscles in the group NB with none blood discharge had obvious extravasated blood, with mediate in the whiteness of the abdominal muscles in the group PB. In summary, the disposal of room temperature water can effectively promote the bleeding of the fish, and significant freshness-locked effect in terms of cold storage stability and sensory quality.
2022, Vol. 37 
