Artificial propagation and embryonic and larval development of Chinese hook snout carp Opsariichthys bidens
LUO Xiaonian, DUAN Youjian*, ZHANG Xiaojing, ALIDA·Xiamuxiding,LI Jiao, WU Chen, LI Wenkang, JI Chenyue, CHANG Jiantai
1.College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China; 2.Key Laboratory of Fish Applied Biology and Aquaculture in North China, Liaoning Province,Dalian 116023, China
Abstract: In order to realize the whole process of artificial propagation and culture of wild Chinese hook snout carp Opsariichthysbidens, the fish was induced to spawn by hormone injection, the fertilized eggs were incubated and fry and juveniles were reared in a pond at Xingda Farm in Liaoyang County, Liaoning Province from 2018 to 2021.Meanwhile embryonic and larval development was observed under a microscope. It was found that male fish were maturity at 1+ year old, and the female at 2+ years old in a pond culture. The pond-cultured Chinese hook snout carp spawned from May 10th to May 29th in Liaoyang area, and had average success rate of 73.8%, fertilization rate of 81.3% and hatching rate of 78.8% by injection of mixed drugs (LHRH-A2 4-8 μg/kg+DOM 4-8 mg/kg+HCG 1 000-2 000 IU/kg), artificial dry insemination, combined hatching basket and hatching bucket incubation method. The fertilized eggs of Chinese hook snout carp are orange-yellow in color without adhesion, and the spawners had effect time of 25 to 27 h after hormone injection at water temperature from 18 to 20 ℃ (one injection). The embryonic development was divided into 7 stages including blastoderm, cleavage, blastula, gastrula, neuroblastoma, organogenesis, and hatching and 24 sub-stages, lasting about 62 h 6 min, with total incubation accumulative temperature of 1 199.91 ℃·h. The larvae swam horizontally over 5 to 6 days post hatching, and the 1-day-old Chinese hook snout carp larvae had total length of (5.13±0.63)mm. The larvae became the mixed nutrition period at the 10-day-old age, and the 12-day-old postlarvae had exhaustion of the yolk sac, and allometric growth. Total 14 000 F1 fry were obtained in the ponds in 2018, and 2 120 000 F2 fry were reared for two consecutive years from 2020 to 2021. The findings realized the whole procedure of artificial propagation of Chinese hook snout carp for the first time.
2022, Vol. 37 
