Artificial propagation technology, and embryonic and early larval development of Schizothorax irregularis in Kizil River in Xinjiang
ZHAO He1,2,3, HONG Jibiao1,2,3, ZHAO Nianhua1,2,3, HU Renyun4, WEI Jie1,2,3*, NIE Zhulan1,2,3
1.College of Animal Sciences, Tarim University, Aral 843300, China; 2.Key Laboratory of Tarim Animal Husbandry Science and Technology, Xinjiang Production & Construction, Aral 843300, China; 3.Tarim Rare Fish Research Center, Aral 843300, China; 4.Wuhan Zhongke Ruihua Ecological Technology Company Limited, Wuhan 430000, China
Abstract: In this study, key artificial propagation techniques including spawning season, sex identification, hormone injection and incubation methods were investigated in 1 380 individuals of Schizothoraxirregulari with body length of (152.31-271.25)mm and body mass of (102.17-265.31)g collected from Kizil River in Xinjiang in December 2017 and from January to December 2018 to accomplish farming and stock enhancement of the animals. A total of 35 pairs of 3 years old male and 4 years old female spawners at a ratio of female to male = 1∶2 was induced to spawn from late April to middle May at water temperature from 16.0 ℃ to 19.5 ℃ with an interval of 0.5 ℃ by injection of luteinizing hormone releasing hormone analogue (LHRH-A3)(3 mg/kg+4 mg/kg), dioxone maleate (DOM) (3 mg/kg+3 mg/kg) and human chorionic gonadotropin (HCG)(1 000 IU/kg). A total of 206 900 eggs and 141 200 postlarvae were obtained, with the optimum water temperature of 18.5 ℃ for induced spawn and 17.0-17.5 ℃ for incubation in the Southern of Xinjiang Uyghur Autonomous Region. S.irregularis had the eggs with diameter of (2.95±0.15)mm, and (3.53±0.12)mm after water absorption. The embryonic development was divided into seven stages: blastoderm, cleavage, blastula, gastrula, neuroblast, organogenesis and hatching. The embryos were hatched in 147 h 31 min at water temperature of (17.14±0.01)℃, with total accumulative temperature of 2 513.01 ℃·h. The newly hatched larvae had total length of (8.72±0.26)mm, staying at the bottom of the water and occasionally moved about in most of the period, even with heart beating and blood circulation. The development of the early larvae was clasified into four stages: eye pigmentation increasing period, body pigmentation increasing period, air bladder inflation period, and yolk sac exhausted completely. The larvae had completely exhausted yolk sac in 432 h, and the total length of (21.51±0.75)mm. The findings help to understand the developmental characteristics of embryos and early larvae of S.irregularis, so as to further enrich the early life history data and provide scientific basis for resource protection and artificial propagation of S.irregularis.
赵贺, 洪继彪, 赵年桦, 胡仁云, 魏杰, 聂竹兰, . 新疆克孜勒河厚唇裂腹鱼人工繁殖及胚胎、早期仔鱼发育研究[J]. 大连海洋大学学报, 2021, 36(3): 414-422.
ZHAO He, HONG Jibiao, ZHAO Nianhua, HU Renyun, WEI Jie, NIE Zhulan, . Artificial propagation technology, and embryonic and early larval development of Schizothorax irregularis in Kizil River in Xinjiang. Journal of Dalian Ocean University, 2021, 36(3): 414-422.
2021, Vol. 36 
