Reproductive toxicity and transgenerational effects of di(2-ethylhexyl)phthalate (DEHP) on water fleas Daphnia magna
WEI Jie1, SHEN Chen-chen1, ZHAO Wen1, WANG Yuan1, BAN Yan-li1, SHEN Qi1, WANG Tian-yi1, XIE Xi2
1.Key Laboratory of Hydrobiology in Liaoning Province, Dalian Ocean University, Dalian 116023, China; 2.Liaoning Open Laboratory of Applied Marine Biology, Liaoning Ocean and Fisheries Science Research Institute, Dalian 116023, China
Abstract: The acute and chronic toxicities of di(2-ethylhexyl)phthalate (DEHP) to water fleas Daphniamagna were investigated in DEHP concentration of 0 (as control group, CK ), 0.4, 0.6, 0.8, 1.0, 1.2 and 1.4 mg/L in acute toxicity and of 0, 0.03, 0.04, 0.05, 0.06, and 0.07 mg/L in chronic toxicity (acetone solvent as control group, CK-S) at water temperature of (23±1)℃ by experimental ecology methods to evaluate the effects of DEHP on fecundity and population growth of three generations (P, F1 and F3) of the water fleas.In acute toxicity test, the LC50 of DEHP was found to be 0.820 mg/L in 24 h and 0.569 mg/L in 48 h with safety concentration of 0.082 mg/L, suggesting that DEHP was a highly toxic chemical compound.The chronic toxicity test revealed that somewhat toxic effects on distinct generations of offspring were observed at DEHP concentration ranging from 0.03 to 0.07 mg/L.The positive improvement of total number of neonates, average number of neonates per brood, first breeding interval, and average interval between broods and intrinsic population growth of the P generation was found at 0.06-0.07 mg/L of DEHP, without distinct effect in the F1 generation.In the F3 generation, however, the total number of neonates, average number of neonates per brood and intrinsic population growth were to some extent inhibited by DEHP.The findings provide basic information for safety assessment of phthalates.
2019, Vol. 34 
