Abstract: Tourmaline(T), polyethylene (PE) and expandable polystyrene(EPS) foam filter beads were disposed in a PVC circulating water system and microbial community structure and evolution were investigated in these systems by Illumina Miseq high-throughput sequencing technology in order to explore the connection among biofilm, microbial diversity and water treatment effect by different filter materials. The results showed that the elimination time of ammonia nitrogen and nitrite was found to be 4 days earlier in tourmaline group than in EPS foam filtration group and 8 days earlier than in PE group. During the water treatment effect test, the order of removal efficiency of ammonia nitrogen and nitrite nitrogen was expressed as tourmaline>PE>EPS foam within 48 h. The analysis of bacterial microbial richness and diversity revealed that the higher microbial richness and diversity bacteria were Proteobacteria, Firmicutes, Bacteroidetes, Actinobacteria, and Nitrospirae in phylum level in the circulating water of each group, in which Proteobacteria and Nitrospirae had important denitrification functions. The analysis of bacterial composition in filter materials in waters indicated that there was significantly higher Proteobacteria in the water in tourmaline group than that in PE group, and that Proteobacteria and Nitrospira in filter media were significantly higher in tourmaline group than in PE and EPS groups. To sum up, by comparing the film mounting time and water treatment effect, it can be concluded that the water treatment effect of tourmaline was superior to PE and EPS foam filter beads, indicating that the deformation bacteria and nitrifying spiral bacteria played an important denitrification function in the water treatment effect.
章霞, 徐志进, 柳敏海, 李伟业, 殷小龙. 循环水养殖系统中不同滤料生物挂膜水处理效果及微生物群落分析[J]. 大连海洋大学学报, 2020, 35(4): 528-535.
ZHANG Xia, XU Zhijin, LIU Minhai, LI Weiye, YIN Xiaolong. Analysis of biofilm water treatment effect and microbial community on different filter materials in a circulating aquaculture system. Journal of Dalian Ocean University, 2020, 35(4): 528-535.
2020, Vol. 35 
