Journal of Dalian Ocean University  2024, Vol. 39 Issue (1): 57-65 DOI: 10.16535/j.cnki.dlhyxb.2023-168 |
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| Effects of substrate material and particle size on survival, growth and microbial community of whelk (Babylonia areolata) |
| HUANG Ruipin，ZHANG Lei，ZHANG Weixiao，LIU Mingyuan，SUI Changrun，WANG Baolong，LIU Ying，ZHANG Qian*
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| 1.Key Laboratory of Environment Controlled Aquaculture (Dalian Ocean University)，Ministry of Education，Dalian 116023，China；2.College of Marine Science and Environment，Dalian Ocean University，Dalian 116023，China；3.College of Life Science，Liaoning Normal University，Dalian 116081，China；4.College of Biological Systems Engineering and Food Science，Zhejiang University，Hangzhou 310058，China |
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Abstract To explore the appropriate substrate for outdoor cement pond cultivation of whelk (Babylonia areolata), the whelk with body weight of (4.692±1.033)g were reared in a rectangular culture pond of 3.6 m in length×1.4 m in width×1.0 m in height with 12 cm-high plastic screw frame covered with a 245 μm sieve silk mesh at bottom of the pond at stocking density of 480 individuals/m3 at water temperature of (30.2±2.7)℃. Sea sand (group SS), fine ceramsite with particle size of 1.0-2.0 mm (group FC), coarse ceramsite with particle size of 2.0-4.0 mm (group CC) and sea sand (50%)+ coarse ceramsite (50%)mixture (group SS+CC) were disposed on the 245 μm sieve silk mesh at a thickness of 5 cm in the test ponds to investigate effects of substrate material and particle size on the survival and growth of B.areolata, water quality and substrate microbial community. The results showed that there was over 90% of survival rate in B.areolata under the four sediment conditions, without significant difference among groups(P>0.05). The maximal specific weight growth rate was observed in group FC (1.386 4 %/d±0.219 6 %/d), significantly higher than that in groups CC, SS and SS+CC (P<0.05). The structure and composition of microbial community were varied with different substrates, with the minimal richness and diversity in group SS, and higher richness and diversity in group FC. There were higher relative abundances of Proteobacteria, Planctomycetes, Bacteroidetes and Acidobacteriota in group FC at phylum level, with lower relative abundance of Vibrio and higher relative abundance of Exiguobacterium and Halomonas in both group FC and group SS+CC. The fine ceramsite had positive effect on resisting pathogenic Vibrio and preventing water quality deterioration. The results of microbial function prediction indicated an increase in relative abundance of pathways associated with various microbial substance metabolisms in the group FC. Also, the substrate led to affect the water quality during aquaculture, with lower concentration of  in group CC than that in group FC (P<0.05). The findings indicated that utilization of fine ceramsite as the substrate material in outdoor cement ponds for B.areolata cultivation promoted body weight growth, inhibited the growth of pathogenic microorganisms, and contributed to the water purification in aquaculture ponds at certain level.
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Published: 13 March 2024
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