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Abstract In order to investigate the protective effect of phillygenin (PHI) on liver injury induced by subchronic toxic concentration of microcystins (MCs), loach (Paramisgurnus dabryanus) with body weight of (100.13±5.23)g were intraperitoneally injected with 17 μg/kg (body weight) MCs on the first day, and then successively injected with different concentrations of PHI [cumulative dose of 0.3, 0.6, 1.2 and 2.4 mg/kg (body weight)] on the following 3 days. On the sixth day, livers of P.dabryanus were taken to compare the histopathologic changes, alanine aminotransferase (ALT) activity, oxidative damage related indices [malonaldehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), glutathione reductase (GR)] and inflammatory factors [interleukin 1β (IL-1β) and tumor necrosis factor α (TNF-α)] of liver tissue. It was found that MCs induced histopathologic injury in liver. There was significant increase in ALT activity, the contents of MDA, and IL-1β and (TNF-α) 2 inflammatory factors in MCs group compared with the control group (P<0.05), while there was significant decrease in activities of 3 antioxidant enzymes in MCs group (P<0.05). The injection of PHI alleviated the MCs-induced histopathologic injury in liver, significantly decreased ALT activity, MDA content and 2 inflammatory factors levels (P<0.05), and significantly increased in the activities of SOD, GSH-PX and GR (except 0.3 mg/kg PHI) (P<0.05) compared with the MCs group. PHI alleviated the histopathologic and physiology function injury, oxidative damage and inflammatory response in liver, and had a protective effect on liver injury induced by MCs.
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Published: 18 January 2024
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2023, Vol. 38 
