Journal of Dalian Ocean University  2023, Vol. 38 Issue (6): 935-946 DOI: 10.16535/j.cnki.dlhyxb.2023-073 |
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| Screening of SNP locus and association with growth traits of MYF5 gene in Nile tilapia (Oreochromis niloticus) |
| TONG Yannan，GAO Fengying*，LIU Zhigang，WANG Miao，CAO Jianmeng，YI Mengmeng，KE Xiaoli，LU Maixin，ZHU Hai*
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| 1.Hainan Academy of Ocean and Fisheries Science，Haikou 570206，China；2.Key Laboratory of Tropical and Subtropical Fishery Resource Application and Cultivation，Ministry of Agriculture and Rural Affairs，Pearl River Fisheries Research Institute，Chinese Academy of Fishery Sciences，Guangzhou 510380，China |
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Abstract To explore the correlation between single nucleotide polymorphisms (SNP) markers of the MYF5 gene and the growth traits of Nile tilapia (Oreochromis niloticus), SNP loci were screened through PCR and sequencing, and confirmed through sequencing methods. Thirty SNP loci with high polymorphism were selected from 35 loci for genotyping analysis in the Gaoyao parent population, and association analysis between SNP loci and growth-related traits was conducted. The obtained growth related loci in Gaoyao broodstock population were verified in Gaoyao offspring population and Panyu populations. Finally, the growth traits related loci obtained above were subjected to genotyping analysis in the Hainan population for further validation. The results showed that S18 (G-1739C) was correlated with body weight and body width, S3 (A-113G) and S4 (C-170T) loci were correlated with total length, and S5 (C-323T) and S24 (C-2345T) were correlated with head length, in the Gaoyao broodstock population. In the Gaoyao offspring population, S3 and S4 loci were related to body weight; S24 was related to body length and body height. Three SNP loci related with body weight were obtained in the Panyu population, namely S7 (A-626T), S21 (C-2090G), and S32 (G-2759T), with one locus S7 related with total-length. No growth-trait related loci were found in the Hainan population. The analysis of the correlation between diplotype and growth traits in various populations showed that one diplotype significantly correlated with total length, one diplotype significantly correlated with head length, and one diplotype significantly correlated with body height was obtained in the Gaoyao broodstock population. One diplotype significantly correlated with body weight was obtained in the Gaoyao offspring population. No diploidy related to growth traits was found in the Panyu and Hainan populations.The findings suggest that S3, S4 and S18 SNP loci obtained from the MYF5 gene in this study might link or be closely related to the growth performance of Nile tilapia. These loci can be used as candidate gene markers for growth related molecular marker assisted breeding of Nile tilapia.
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Published: 18 January 2024
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| [1] |
LIU Zhigang, GAO Fengying, TONG Yannan, YI Mengmeng, WANG Miao, ZHU Hai, LU Maixin, KE Xiaoli, CAO Jianmeng. Screening and verification of microsatellite markers associated with growth traits in Nile tilapia (Oreochromis niloticus)[J]. Journal of Dalian Ocean University, 2023, 38(6): 925-934. |
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