Journal of Dalian Ocean University  2022, Vol. 37 Issue (5): 732-738 DOI: 10.16535/j.cnki.dlhyxb.2021-217 |
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| Effects of dietary vitamin C on reproduction performance and offspring larval quality of goldfish Carassius auratus |
| LI Tieliang, XING Wei, XU Guanling, MA Zhihong, JIANG Na, YU Huanhuan, LUO Lin*
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| Fisheries Research Institute, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100068, China |
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Abstract Ten female and twenty male broodstock goldfish Carassius auratus with body weight of (6.60±0.12)g (♀1∶♂2) were reared in a 1.5 m×1.5 m×0.6 m tank, fed diets containing 0.175 (control group), 0.875, 1.75 and 3.50 mg/g L-ascorbate-2-phosphate (vitamin C, VC) at water temperature of 20 ℃ for 3 months, and then artificial reproduction was conducted to investigate the effects of VC on reproduction performance and offspring quality. The results showed that the absolute fecundity of female goldfish and the sperm concentration of male goldfish were significantly affected by the dietary level of VC (P<0.05), significant increase in the eyed rate, hatching rate and survival rate of 4-day old larvae in the broodstock fish fed the diet with supplementation of 1.75 mg/g VC (P<0.05). There were significantly higher non-specific immune factors including complement C3 levels in serum and antioxidant capacity in ovary and testis of brood stock fed the diet with supplementation of VC (P<0.05). In conclusion, the high dose (0.875 to 1.75 mg/g) VC supplementation in the diet leads to improve the reproductive performance of goldfish broodstocks, inluding gonad secretion, quantity and quality of sperm and eggs, and offspring quality under the experimental conditions.
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Published: 14 November 2022
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