Journal of Dalian Ocean University  2019, Vol. 34 Issue (1): 15-20 DOI: 10.16535/j.cnki.dlhyxb.2019.01.003 |
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| Optimal fermentation medium and growth in a fermentor of Pseudomonas aeruginosa inactivated vaccine in grass carp |
| SUN Cheng-wen1, LAI Ying-tiao1, REN Xiao-bo2, JIANG Xiao-yan1, GONG Hua1, HUANG Zhi-bin1, TAO Jia-fa1
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| 1.Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China; 2.College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China |
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Abstract The nitrogen source, carbon source and phosphate were screened in culture medium of Pseudomonas aeruginosa inactivated vaccine in a single-factor test, and different ingredients of the medium were optimized in an orthogonal experiment, with a verification test of growth of the bacterium in a 5 L fermentor.It was found that the best fermentation of Pseudomonas aeruginosa in grass carp Ctenopharyngodon idellus with red skin disease was observed under the conditions of peptone+beef extract+yeast extract as nitrogen source, glucose as carbon source, K2PHO4 as phosphate source, at a dose of peptone 10 g/L, beef extract 5.0 g/L, yeast extract 2.5 g/L, glucose 5.0 g/L, K2HPO4 0.75 g/L, and NaCl 5.0 g/L.The fermentation kinetics analysis revealed that the stain had the maximal cell density at 6.44 of OD600 nm in 14 h culture, indicating that a high yield of Pseudomonas aeruginosa JP802 fermentation broth is obtained by the optimized fermentation medium.
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Published: 05 May 2019
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