Inhibition of α-glucosidase activity and hypoglycemic effect by fucoxanthin extracted from seaweed (Sargassum horneri)
LIU Yan, ZHI Lichao, WANG Huirui, ZHAO Li, REN Dandan*, HE Yunhai, WANG Qiukuan
1.College of Food Science and Engineering, Dalian Ocean University, Dalian 116023, China； 2.Shandong Jiejing Group Company Limited, Rizhao 276800, China； 3.Nation Research and Development Branch Center For Seaweed Processing, Dalian 116023, China； 4.Collaborative Innovation Center of Seafood Deep Processing (Dalian Polytechnic University), Dalian 116034, China
Abstract: To investigate the effect of fucoxanthin on blood glucose and possible mechanism in type Ⅱ diabetic mice, the blood glucose and lipid metabolism related indices were determined in the type Ⅱ diabetes model mice established by streptozotocin (STZ). The type Ⅱ diabetes model mice was gavaged with fucoxanthin extracted from seaweed (Sargassumhorneri) at a dose of 25, 50 and 100 mg/kg and the inhibition effect of fucoxanthin on α-glucosidase was investigated, and the pathological changes were observed in liver tissues of the mice by Hematoxylin-Eosin (HE) staining. The mRNA relative expression levels of cellular PI3K/AKT signaling pathway indicators including phosphatidylinositol 3-kinase (PI3K), and protein kinase B (AKT), glycogen synthase (GS) and glucose transporter 4 (GLUT4) were detected by fluorescence quantitative PCR. The results showed that the fucoxanthin had inhibition rate of 76.43%±0.83% on α-glucosidase, with the noncompetitive inhibition type, resulting in significantly decrease in the levels of fasting blood glucose (FBG), insulin (INS), total cholesterol (TC), triglyceride (TG) and low density lipoprotein (LDL-C), and in increase in the content of liver glycogen and high density lipoprotein (HDL-C) in the diabetic model mice. Histopathological observation showed that the infiltration of liver inflammatory cells was relieved by the fucoxanthin. There was increase in the mRNA expression levels of PI3K, AKT, GS and GLUT4 mRNA in diabetic mice orally administrated by fucoxanthin. The findings indicated that fucoxanthin regulated blood glucose in mice through PI3K/AKT signaling pathway with dose-dependent relationship, and the best hypoglycemic and hypolipidemic effects was at 100 mg/kg.
2023, Vol. 38 
