Tooth development and mineralization in mandarin fish (Siniperca chuatsi) at early stage
CAO Linjun, ZHAO Jinliang*, XU Jun, ZHANG Qi, CHEN Ming
Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China
Abstract: In order to understand tooth development and mineralization process of mandarin fish (Sinipercachuatsi), the changes in tooth occurrence time, number and column number were observed from 1 day old to 55 days old larva and juveniles after hatching by anatomical microscope and light microscopy at water temperature from 23 ℃ to 25 ℃. The tooth mineralization time was observed by cartilage bone staining and Von Kossa staining, and the contents of calcium and phosphorus were determined during tooth mineralization by X-ray energy spectrometer. The changes in alkaline phosphatase protein expression were observed by immunohistochemical method, and the expression levels of connexin 43 (Cx43) and Calbindin-d28k (CaB) were detected by fluorescence quantitative PCR. The results showed that the maxillary tooth and mandibular tooth were found at 3 days post hatch (dph), the pharyngeal tooth at 5 dph, the palate tooth at 8 dph and the vomerine tooth at 12 dph. The mandibular dentition were fully developed at 8 dph, while maxillary dentition at 11 dph. In the maxillary and mandibular tooth, villous tooth developed firstly, canine tooth began to develop at 18 dph, with completely mineralized teeth from 11 dph to 14 dph. During the tooth mineralization, the contents of Ca and P were shown to be increased significantly (P<0.05), without significant difference in Ca/P (P>0.05). Positive reaction was detected in the enamel and dentin at 10 dph and 12 dph. Relative expression levels of Cx43 and CaB genes were increased at 10 dph and 12 dph, with a significant increase for CaB gene (P<0.05), and significant decrease in expression levels of both genes at 14 dph (P<0.05). The findings indicate that the early teeth of the mandarin fish can directly bite and fix live baitfish to prevent them from escaping. In the process of tooth mineralization, calcium, phosphorus and mineralization-related proteins are all involved.
曹林军, 赵金良, 徐俊, 张琦, 陈铭. 鳜仔稚鱼牙齿发育和矿化过程观察[J]. 大连海洋大学学报, 2023, 38(1): 51-58.
CAO Linjun, ZHAO Jinliang, XU Jun, ZHANG Qi, CHEN Ming. Tooth development and mineralization in mandarin fish (Siniperca chuatsi) at early stage. Journal of Dalian Ocean University, 2023, 38(1): 51-58.
2023, Vol. 38 
